Detailed information NTG0312416

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: E4U63_RS01305
• Genome accession: NZ_CP038344
• Coordinates: 273460..274443 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain Gim1-1
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 268460..279443

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4U63_RS01290 (E4U63_01340)	dppA	269760..271367 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
E4U63_RS01295 (E4U63_01350)	dppB	271518..272537 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
E4U63_RS01300 (E4U63_01355)	dppC	272547..273449 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
E4U63_RS01305 (E4U63_01360)	amiE	273460..274443 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
E4U63_RS01310 (E4U63_01365)	dppF	274440..275444 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
E4U63_RS01315 (E4U63_01370)	yhjV	275474..276745 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
E4U63_RS01320 (E4U63_01375)	ldrD	277221..277328 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
E4U63_RS01325 (E4U63_01380)	bcsG	277415..279094 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
E4U63_RS01330 (E4U63_01385)	bcsF	279091..279282 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=312416 E4U63_RS01305 WP_001196486.1 273460..274443(+) (amiE) [Escherichia coli O157:H7 strain Gim1-1]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=312416 E4U63_RS01305 WP_001196486.1 273460..274443(+) (amiE) [Escherichia coli O157:H7 strain Gim1-1]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404