Detailed information    

insolico Bioinformatically predicted

Overview

Name   recR   Type   Machinery gene
Locus tag   ECBD_RS16645 Genome accession   NC_012947
Coordinates   3318328..3318933 (-) Length   201 a.a.
NCBI ID   WP_001195025.1    Uniprot ID   P0A7H7
Organism   Escherichia coli 'BL21-Gold(DE3)pLysS AG'     
Function   promote later steps in plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3313328..3323933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECBD_RS16625 (ECBD_3180) aes 3313365..3314324 (+) 960 WP_000801849.1 acetyl esterase -
  ECBD_RS16630 (ECBD_3181) hemH 3314321..3315283 (-) 963 WP_001250117.1 ferrochelatase -
  ECBD_RS16635 (ECBD_3182) adk 3315519..3316163 (-) 645 WP_001220233.1 adenylate kinase -
  ECBD_RS16640 (ECBD_3183) htpG 3316344..3318218 (-) 1875 WP_000678201.1 molecular chaperone HtpG -
  ECBD_RS16645 (ECBD_3184) recR 3318328..3318933 (-) 606 WP_001195025.1 recombination mediator RecR Machinery gene
  ECBD_RS16650 (ECBD_3185) ybaB 3318933..3319262 (-) 330 WP_000467098.1 YbaB/EbfC family nucleoid-associated protein -
  ECBD_RS16655 (ECBD_3186) dnaX 3319315..3321246 (-) 1932 WP_000122013.1 DNA polymerase III subunit gamma/tau -
  ECBD_RS16660 (ECBD_3187) apt 3321375..3321926 (-) 552 WP_000127356.1 adenine phosphoribosyltransferase -
  ECBD_RS16665 (ECBD_3188) ybaN 3322079..3322456 (-) 378 WP_001188905.1 DUF454 family protein -
  ECBD_RS16670 (ECBD_3189) priC 3322526..3323053 (+) 528 WP_000844874.1 primosomal replication protein N'' -
  ECBD_RS16675 (ECBD_3190) rsmS 3323067..3323228 (+) 162 WP_000051153.1 pleiotropic regulatory protein RsmS -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 21963.00 Da        Isoelectric Point: 4.9097

>NTDB_id=31198 ECBD_RS16645 WP_001195025.1 3318328..3318933(-) (recR) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLAQALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQEN
GQICVVESPADIYAIEQTGQFSGRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF

Nucleotide


Download         Length: 606 bp        

>NTDB_id=31198 ECBD_RS16645 WP_001195025.1 3318328..3318933(-) (recR) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCG
TATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAA
TCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAAC
GGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGT
GTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAG
AGGAAAAAATCACTGAAGTGATCCTCGCCACCAATCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTT
TGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGATGGCAC
CACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAA

Domains

Predicted by InterProScan.

RecR protein (RecR_ZnF)

(41-77)

Toprim domain (Toprim_4)

(81-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A7H7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recR Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.226

99.005

0.448
  recR Bacillus subtilis subsp. subtilis str. 168

44.724

99.005

0.443

Multiple sequence alignment