Detailed information NTG0031166

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ECBD_RS05075
• Genome accession: NC_012947
• Coordinates: 1032385..1033377 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1027385..1038377

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECBD_RS05045 (ECBD_0977)	ispD	1027446..1028156 (+)	711	WP_000246144.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
ECBD_RS05050 (ECBD_0978)	ispF	1028156..1028635 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ECBD_RS05055 (ECBD_0979)	truD	1028632..1029681 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
ECBD_RS05060 (ECBD_0980)	surE	1029662..1030423 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ECBD_RS05065 (ECBD_0981)	pcm	1030417..1031043 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ECBD_RS05070 (ECBD_0982)	nlpD	1031228..1032322 (+)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
ECBD_RS05075 (ECBD_0983)	rpoS	1032385..1033377 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
ECBD_RS05080 (ECBD_0984)	ygbN	1033471..1034835 (-)	1365	WP_000104459.1	GntP family transporter	-
ECBD_RS05085 (ECBD_0985)	ygbM	1034924..1035700 (-)	777	WP_001136925.1	HPr family phosphocarrier protein	-
ECBD_RS05090 (ECBD_0986)	ygbL	1035705..1036343 (-)	639	WP_001278994.1	aldolase	-
ECBD_RS05095 (ECBD_0987)	otnK	1036340..1037602 (-)	1263	WP_000590371.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=31166 ECBD_RS05075 WP_000081550.1 1032385..1033377(+) (rpoS) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=31166 ECBD_RS05075 WP_000081550.1 1032385..1033377(+) (rpoS) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715