Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ECBD_RS00465 Genome accession   NC_012947
Coordinates   93523..94191 (+) Length   222 a.a.
NCBI ID   WP_001321683.1    Uniprot ID   -
Organism   Escherichia coli 'BL21-Gold(DE3)pLysS AG'     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 88523..99191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECBD_RS00435 (ECBD_0082) yicC 88542..89405 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  ECBD_RS00440 (ECBD_0083) rph 89532..90248 (+) 717 WP_001247089.1 ribonuclease PH -
  ECBD_RS00445 (ECBD_0084) pyrE 90314..90955 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  ECBD_RS00450 (ECBD_0085) slmA 90992..91588 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  ECBD_RS00455 (ECBD_0086) dut 91695..92153 (-) 459 WP_000976070.1 dUTP diphosphatase -
  ECBD_RS00460 (ECBD_0087) coaBC 92131..93351 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ECBD_RS00465 (ECBD_0088) radC 93523..94191 (+) 669 WP_001321683.1 DNA repair protein RadC Machinery gene
  ECBD_RS00470 (ECBD_0089) rpmB 94408..94644 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  ECBD_RS00475 (ECBD_0090) rpmG 94665..94832 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  ECBD_RS00480 (ECBD_0091) mutM 94930..95739 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ECBD_RS00485 (ECBD_0092) coaD 95778..96257 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  ECBD_RS00490 (ECBD_0093) waaA 96265..97542 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ECBD_RS00495 (ECBD_0094) rfaQ 97955..99013 (+) 1059 WP_000360401.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25257.41 Da        Isoelectric Point: 8.9890

>NTDB_id=31148 ECBD_RS00465 WP_001321683.1 93523..94191(+) (radC) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGKYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=31148 ECBD_RS00465 WP_001321683.1 93523..94191(+) (radC) [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAAAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment