Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D0819_RS17460 Genome accession   NZ_CP031784
Coordinates   3354391..3355767 (+) Length   458 a.a.
NCBI ID   WP_015252992.1    Uniprot ID   A0A0C3LIP7
Organism   Bacillus subtilis strain HMNig-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3349391..3360767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0819_RS17440 (D0819_17515) ctsR 3349744..3350208 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  D0819_RS17445 (D0819_17520) mcsA 3350222..3350779 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  D0819_RS17450 (D0819_17525) mcsB 3350779..3351870 (+) 1092 WP_021482008.1 protein arginine kinase -
  D0819_RS17455 (D0819_17530) clpC 3351867..3354299 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  D0819_RS17460 (D0819_17535) radA 3354391..3355767 (+) 1377 WP_015252992.1 DNA repair protein RadA Machinery gene
  D0819_RS17465 (D0819_17540) disA 3355771..3356853 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  D0819_RS17470 (D0819_17545) yacL 3356969..3358069 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  D0819_RS17475 (D0819_17550) ispD 3358084..3358782 (+) 699 WP_064672086.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D0819_RS17480 (D0819_17555) ispF 3358775..3359251 (+) 477 WP_014475594.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49438.94 Da        Isoelectric Point: 8.2399

>NTDB_id=311416 D0819_RS17460 WP_015252992.1 3354391..3355767(+) (radA) [Bacillus subtilis strain HMNig-2]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=311416 D0819_RS17460 WP_015252992.1 3354391..3355767(+) (radA) [Bacillus subtilis strain HMNig-2]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACC
CGCGGCAAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3LIP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.563

100

0.996
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment