Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D0819_RS08865 Genome accession   NZ_CP031784
Coordinates   1684286..1684696 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain HMNig-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1679286..1689696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0819_RS08835 (D0819_08850) - 1679372..1679512 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  D0819_RS08840 (D0819_08855) yqeF 1679818..1680549 (-) 732 WP_153256925.1 SGNH/GDSL hydrolase family protein -
  D0819_RS08845 (D0819_08860) cwlH 1680801..1681553 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  D0819_RS08850 (D0819_08865) yqeD 1681740..1682366 (+) 627 WP_038829700.1 TVP38/TMEM64 family protein -
  D0819_RS08855 (D0819_08870) gnd 1682386..1683279 (-) 894 WP_153256926.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  D0819_RS08860 (D0819_08875) yqeB 1683531..1684220 (+) 690 WP_153256927.1 DUF308 domain-containing protein -
  D0819_RS08865 (D0819_08880) nucA/comI 1684286..1684696 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  D0819_RS08870 (D0819_08885) sigK 1684892..1685620 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  D0819_RS08875 - 1685620..1685736 (+) 117 WP_070547560.1 hypothetical protein -
  D0819_RS08880 (D0819_08890) - 1685702..1685899 (-) 198 Protein_1767 recombinase family protein -
  D0819_RS08885 (D0819_08895) - 1685857..1686010 (-) 154 Protein_1768 hypothetical protein -
  D0819_RS08890 (D0819_08900) psiE 1686551..1686967 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  D0819_RS08895 (D0819_08905) yrkQ 1687013..1688311 (-) 1299 WP_153256928.1 two-component system sensor histidine kinase YrkQ -
  D0819_RS08900 (D0819_08910) yrkP 1688298..1688993 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=311384 D0819_RS08865 WP_009967785.1 1684286..1684696(-) (nucA/comI) [Bacillus subtilis strain HMNig-2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=311384 D0819_RS08865 WP_009967785.1 1684286..1684696(-) (nucA/comI) [Bacillus subtilis strain HMNig-2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment