Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   D0808_RS08375 Genome accession   NZ_CP031783
Coordinates   1628800..1632498 (+) Length   1232 a.a.
NCBI ID   WP_153255881.1    Uniprot ID   -
Organism   Bacillus subtilis strain MENO2 voucher National Research Centre     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1623800..1637498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0808_RS08360 (D0808_08455) cusC 1624249..1624575 (-) 327 WP_153255879.1 four-helix bundle copper-binding protein -
  D0808_RS08365 (D0808_08460) yhjR 1624692..1625129 (-) 438 WP_003245806.1 ferritin family protein -
  D0808_RS08370 (D0808_08465) addB 1625313..1628813 (+) 3501 WP_153255880.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D0808_RS08375 (D0808_08470) addA 1628800..1632498 (+) 3699 WP_153255881.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D0808_RS08380 (D0808_08475) sbcD 1632570..1633745 (+) 1176 WP_086343673.1 exonuclease subunit SbcD -
  D0808_RS08385 (D0808_08480) sbcC 1633742..1637134 (+) 3393 WP_153255882.1 exonuclease subunit SbcC -
  D0808_RS08390 (D0808_08485) hlpB 1637148..1637450 (+) 303 WP_015252406.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 141033.36 Da        Isoelectric Point: 5.0695

>NTDB_id=311292 D0808_RS08375 WP_153255881.1 1628800..1632498(+) (addA) [Bacillus subtilis strain MENO2 voucher National Research Centre]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDGYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGECEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQARTYLDFIGPALARHRDLEDLAGVPAHADISGHPARFAVQMIHSYDLL
DDDLEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG
SILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL
IGAKWKDREIPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQ
IQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=311292 D0808_RS08375 WP_153255881.1 1628800..1632498(+) (addA) [Bacillus subtilis strain MENO2 voucher National Research Centre]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTTTCTCTTTTGAACCGGGCCAGCATTTC
GACGCTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGTGAGCTACTGGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGGATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGTTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCAGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTTGAAAAGCTGAAA
ACCGACTACTTCACGCGAAGTCCTGAACAGCACTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGATTCGAAGCTGCGAAACAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTGAGAATGACAAAGGTGAATGTGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGGCGGAAAATCGATTTAAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACGGTGCAGTTTGAGGCAAAAGCCATCGCTAAGGAAATTCGT
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAAAGAAAACACATCGCAATATTCAATACCGAGATATCGTGAT
TTTGCTCCGTTCGATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATTGATAATCCGTATCAAGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGATGAGCTTTATCAAAAGCTAAATACGT
TTTACGGACATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACCAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGATAGGGC
GCGTCAATATGAATCAACGGCATTTCGCGGCTTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGA
GCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGAGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCATGTAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCCGCATCCCAAACTGATTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAGAACGTATCTAGATTTCATTGGGCCGGCTCTTGCCAGGCACAGAGACTTGGAGGATTTGG
CTGGTGTGCCAGCACACGCTGACATCTCAGGCCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGATTTGCTT
GATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAACGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCCCGGCTC
GTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCTCATCACGAGGTGACGCAAATTCGGACAAAGC
AATCGGTTTCTGAGATCAAAAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAACCGGCTGATGGA
AGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGGACTGCCATGCATACGGT
TATGCAGCATATCCCGCTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCACAGGCTTTATGAAAAAGAGC
TTCTCACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACGGAAATCGGCGGACAGCTG
ATCGGCGCAAAGTGGAAGGACAGGGAAATACCATTCAGCTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGCACAGGA
GGCAGATGAGCCGCTTTTAGTGCAGGGTATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATCTATTGGATTATA
AGTCGGACCGGATTGAGGGCAAATTCCAGCATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAAACGCAA
ATTCAGCTGTACACGAAGGCAGTGGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGACGGAGG
GCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1053-1223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

99.351

100

0.994

Multiple sequence alignment