Detailed information NTG0311159

Bioinformatically predicted

Overview

• Name: ccrB
• Type: Machinery gene
• Locus tag: D1F65_RS00315
• Genome accession: NZ_CP031779
• Coordinates: 61859..63487 (-)
• Length: 542 a.a.
• NCBI ID: WP_001186602.1
• Uniprot ID: Q9XB94
• Organism: Staphylococcus aureus strain CFBR-105
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	43518..64858	61859..63487	within	0

Gene organization within MGE regions

Location: 43518..64858

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D1F65_RS00200 (D1F65_00200)	mecA	43518..45527 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
D1F65_RS00205 (D1F65_00205)	mecR1	45624..47381 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
D1F65_RS00210 (D1F65_00210)	mecI	47381..47752 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
D1F65_RS00215 (D1F65_00215)	psm-mec	47837..47905 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
D1F65_RS00220 (D1F65_00220)	-	48239..49369 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
D1F65_RS00230 (D1F65_00230)	cstB	49483..50817 (-)	1335	Protein_39	persulfide dioxygenase-sulfurtransferase CstB	-
D1F65_RS00235 (D1F65_00235)	-	50850..51914 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
D1F65_RS00240 (D1F65_00240)	cstR	52050..52310 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
D1F65_RS00245 (D1F65_00245)	-	52310..52948 (+)	639	Protein_42	sulfite exporter TauE/SafE family protein	-
D1F65_RS00255 (D1F65_00255)	-	53293..53955 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
D1F65_RS00270 (D1F65_00270)	erm(A)	54464..55195 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
D1F65_RS00275 (D1F65_00275)	-	55321..56103 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
D1F65_RS00280 (D1F65_00280)	-	56254..56631 (-)	378	WP_000361059.1	DUF6262 family protein	-
D1F65_RS00285 (D1F65_00285)	-	56638..58530 (-)	1893	WP_001557544.1	site-specific integrase	-
D1F65_RS00290 (D1F65_00290)	-	58527..59612 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
D1F65_RS00295 (D1F65_00295)	-	59731..60048 (-)	318	WP_000659050.1	JAB domain-containing protein	-
D1F65_RS00300 (D1F65_00300)	-	60069..60575 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
D1F65_RS00305 (D1F65_00305)	-	60593..60904 (-)	312	WP_000700853.1	DUF960 family protein	-
D1F65_RS00310 (D1F65_00310)	-	60991..61341 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
D1F65_RS00315 (D1F65_00315)	ccrB	61859..63487 (-)	1629	WP_001186602.1	recombinase family protein	Machinery gene
D1F65_RS00320 (D1F65_00320)	ccrA	63509..64858 (-)	1350	WP_117374305.1	recombinase family protein	Machinery gene

Sequence

Protein

• Length: 542 a.a.
• Molecular weight: 62664.84 Da
• Isoelectric Point: 9.8600

>NTDB_id=311159 D1F65_RS00315 WP_001186602.1 61859..63487(-) (ccrB) [Staphylococcus aureus strain CFBR-105]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKEGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKPLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide

• Length: 1629 bp

>NTDB_id=311159 D1F65_RS00315 WP_001186602.1 61859..63487(-) (ccrB) [Staphylococcus aureus strain CFBR-105]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGAAGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCATCGGGTAAGCTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGCCATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTTAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGCCTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATAAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTGTTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

• Ogr/Delta-like zinc finger (Ogr_Delta) (301-356)

• Resolvase, N terminal domain (Resolvase) (11-156)

• Recombinase (Recombinase) (180-280)

3D structure

• AlphaFold DB: Q9XB94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrB	Staphylococcus aureus N315	100	100	1