Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   E4U64_RS00825 Genome accession   NZ_CP038287
Coordinates   163056..163724 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain TX 376-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 158056..168724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4U64_RS00795 (E4U64_00825) yicC 158075..158938 (-) 864 WP_000621341.1 YicC/YloC family endoribonuclease -
  E4U64_RS00800 (E4U64_00830) rph 159065..159781 (+) 717 WP_001247093.1 ribonuclease PH -
  E4U64_RS00805 (E4U64_00835) pyrE 159847..160488 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  E4U64_RS00810 (E4U64_00840) slmA 160525..161121 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  E4U64_RS00815 (E4U64_00845) dut 161228..161686 (-) 459 WP_000976070.1 dUTP diphosphatase -
  E4U64_RS00820 (E4U64_00850) coaBC 161664..162884 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  E4U64_RS00825 (E4U64_00855) radC 163056..163724 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  E4U64_RS00830 (E4U64_00860) rpmB 163941..164177 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  E4U64_RS00835 (E4U64_00865) rpmG 164198..164365 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  E4U64_RS00840 (E4U64_00870) mutM 164463..165272 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  E4U64_RS00845 (E4U64_00875) coaD 165311..165790 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  E4U64_RS00850 (E4U64_00880) waaA 165798..167075 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  E4U64_RS00855 (E4U64_00885) rfaQ 167488..168546 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=310991 E4U64_RS00825 WP_001297375.1 163056..163724(+) (radC) [Escherichia coli O157:H7 strain TX 376-2]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=310991 E4U64_RS00825 WP_001297375.1 163056..163724(+) (radC) [Escherichia coli O157:H7 strain TX 376-2]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496