Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   E4T53_RS00825 Genome accession   NZ_CP038282
Coordinates   163037..163705 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain F8492     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 158037..168705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T53_RS00795 (E4T53_00820) yicC 158056..158919 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  E4T53_RS00800 (E4T53_00825) rph 159046..159762 (+) 717 WP_001247093.1 ribonuclease PH -
  E4T53_RS00805 (E4T53_00830) pyrE 159828..160469 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  E4T53_RS00810 (E4T53_00835) slmA 160506..161102 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  E4T53_RS00815 (E4T53_00840) dut 161209..161667 (-) 459 WP_000976070.1 dUTP diphosphatase -
  E4T53_RS00820 (E4T53_00845) coaBC 161645..162865 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  E4T53_RS00825 (E4T53_00850) radC 163037..163705 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  E4T53_RS00830 (E4T53_00855) rpmB 163922..164158 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  E4T53_RS00835 (E4T53_00860) rpmG 164179..164346 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  E4T53_RS00840 (E4T53_00865) mutM 164444..165253 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  E4T53_RS00845 (E4T53_00870) coaD 165292..165771 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  E4T53_RS00850 (E4T53_00875) waaA 165779..167056 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  E4T53_RS00855 (E4T53_00880) rfaQ 167469..168527 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=310833 E4T53_RS00825 WP_001297375.1 163037..163705(+) (radC) [Escherichia coli O157:H7 strain F8492]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=310833 E4T53_RS00825 WP_001297375.1 163037..163705(+) (radC) [Escherichia coli O157:H7 strain F8492]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment