Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BEN71_RS16090 Genome accession   NZ_CP031716
Coordinates   3242338..3243141 (-) Length   267 a.a.
NCBI ID   WP_068973267.1    Uniprot ID   -
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3237338..3248141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS16070 (BEN71_16070) - 3238319..3239062 (-) 744 WP_068973271.1 tetratricopeptide repeat protein -
  BEN71_RS16075 (BEN71_16075) hisS 3239117..3240412 (-) 1296 WP_068973270.1 histidine--tRNA ligase -
  BEN71_RS16080 (BEN71_16080) ispG 3240416..3241534 (-) 1119 WP_068973269.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BEN71_RS16085 (BEN71_16085) - 3241556..3242347 (-) 792 WP_068973268.1 helix-turn-helix domain-containing protein -
  BEN71_RS16090 (BEN71_16090) pilF 3242338..3243141 (-) 804 WP_068973267.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BEN71_RS16095 (BEN71_16095) rlmN 3243201..3244451 (-) 1251 WP_068973266.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BEN71_RS16100 (BEN71_16100) ndk 3244632..3245063 (-) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  BEN71_RS16105 (BEN71_16105) iscX 3245196..3245396 (-) 201 WP_068973265.1 Fe-S cluster assembly protein IscX -
  BEN71_RS16110 (BEN71_16110) - 3245623..3246174 (-) 552 WP_068973264.1 phosphatase PAP2 family protein -
  BEN71_RS16115 (BEN71_16115) - 3246205..3247491 (-) 1287 WP_068973263.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29983.23 Da        Isoelectric Point: 9.5545

>NTDB_id=310691 BEN71_RS16090 WP_068973267.1 3242338..3243141(-) (pilF) [Acinetobacter wuhouensis strain WCHA60]
MYSKAIVMLTIGLFALAIQGCQTTSTGPISKKDPEKAVKVRTQLAAEYIRSGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDPKNAQARNNYGTYLFISERYNDAIEQLSIAGAALGYDQRYKALENLGRVYLKVGD
VVNAEKAFKQALQVNRDSVISLVELSEIFYLQQKNAQARQLYEQYVRLVGQDKQGARALWIGLRIARANQDQMGMQVLVN
QLRALFPDSKEYQRYLQLQNSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=310691 BEN71_RS16090 WP_068973267.1 3242338..3243141(-) (pilF) [Acinetobacter wuhouensis strain WCHA60]
ATGTATTCAAAAGCAATTGTAATGTTGACAATTGGTTTATTCGCATTGGCTATTCAAGGTTGTCAGACGACAAGTACTGG
TCCAATAAGTAAAAAAGATCCAGAGAAAGCCGTCAAAGTACGTACCCAACTTGCTGCAGAATACATCCGTTCAGGTGATT
TGGATGCAGCAAAACGTGCATTAGATCAAGCGCTTGAAACTGATTCACGGGATTCAGCTGCCAATATGATGATGGGTGTT
TTACTGCAACAAGAAGGCAGTAAAATCAGTATGGAAAAAGCAGATGGCTATTTTAAGCGTGCAATTTCTAGTGACCCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTATTAGTGAGCGTTATAATGATGCCATCGAACAGTTGAGCA
TAGCTGGTGCTGCATTAGGATATGATCAGCGTTACAAAGCTTTAGAAAATTTGGGTCGCGTGTATTTGAAGGTAGGCGAT
GTGGTCAATGCTGAAAAAGCATTCAAACAAGCCTTGCAAGTGAATCGCGATTCAGTCATTTCTTTGGTAGAGTTATCTGA
GATTTTTTATTTGCAGCAAAAAAATGCACAAGCACGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGATAAAC
AAGGTGCACGTGCACTTTGGATTGGATTGCGTATTGCACGTGCCAATCAAGACCAGATGGGAATGCAAGTGTTGGTGAAT
CAACTTCGTGCATTATTCCCTGACAGTAAAGAATATCAACGTTATCTGCAATTGCAGAATAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-79)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_16)

(94-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.954

98.127

0.745
  pilF Acinetobacter baylyi ADP1

66.171

100

0.667

Multiple sequence alignment