Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DXY21_RS06490 Genome accession   NZ_CP031694
Coordinates   1272843..1273988 (-) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain SRCM101368     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 1267843..1278988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXY21_RS06440 (DXY21_01283) speE 1268135..1268965 (+) 831 WP_003151052.1 spermidine synthase -
  DXY21_RS06445 (DXY21_01284) speB 1269025..1269897 (+) 873 WP_003151054.1 agmatinase -
  DXY21_RS06450 (DXY21_01285) - 1270067..1270285 (+) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  DXY21_RS06460 (DXY21_01287) - 1270876..1271388 (-) 513 WP_003151059.1 stage II sporulation protein M -
  DXY21_RS06465 (DXY21_01288) - 1271432..1271713 (-) 282 WP_014306021.1 hypothetical protein -
  DXY21_RS06475 (DXY21_01289) - 1272121..1272324 (-) 204 WP_003151062.1 hypothetical protein -
  DXY21_RS06480 (DXY21_01290) - 1272338..1272541 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  DXY21_RS06485 (DXY21_01291) - 1272740..1272859 (-) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  DXY21_RS06490 (DXY21_01292) rapF 1272843..1273988 (-) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  DXY21_RS06495 (DXY21_01293) - 1274280..1274708 (+) 429 WP_003151069.1 DUF1934 domain-containing protein -
  DXY21_RS06500 (DXY21_01294) argS 1274705..1276375 (+) 1671 WP_003151071.1 arginine--tRNA ligase -
  DXY21_RS06505 (DXY21_01295) narK 1276509..1277699 (+) 1191 WP_044052814.1 nitrate transporter NarK -
  DXY21_RS06510 (DXY21_01296) - 1277797..1278513 (+) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=310383 DXY21_RS06490 WP_003151067.1 1272843..1273988(-) (rapF) [Bacillus velezensis strain SRCM101368]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=310383 DXY21_RS06490 WP_003151067.1 1272843..1273988(-) (rapF) [Bacillus velezensis strain SRCM101368]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGTTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53

Multiple sequence alignment