Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DXY22_RS05315 Genome accession   NZ_CP031693
Coordinates   1020819..1021268 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain SRCM101393     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1015819..1026268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXY22_RS05275 - 1016273..1016461 (+) 189 Protein_1005 hypothetical protein -
  DXY22_RS05280 - 1016497..1016812 (+) 316 Protein_1006 RDD family protein -
  DXY22_RS05285 (DXY22_01030) yckD 1016894..1017226 (+) 333 WP_015252849.1 YckD family protein -
  DXY22_RS05290 (DXY22_01031) - 1017354..1017776 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  DXY22_RS05295 (DXY22_01032) - 1017764..1018105 (+) 342 WP_032724911.1 DUF3147 family protein -
  DXY22_RS05300 (DXY22_01034) - 1018124..1018851 (+) 728 Protein_1010 AAA family ATPase -
  DXY22_RS05305 (DXY22_01035) bglC 1018924..1020357 (+) 1434 WP_015252846.1 6-phospho-beta-glucosidase -
  DXY22_RS05310 (DXY22_01036) nin/comJ 1020394..1020792 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  DXY22_RS05315 (DXY22_01037) nucA/comI 1020819..1021268 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  DXY22_RS05320 (DXY22_01038) tlpC 1021436..1023157 (-) 1722 WP_015252843.1 methyl-accepting chemotaxis protein TlpC -
  DXY22_RS05325 (DXY22_01039) hxlB 1023268..1023825 (-) 558 WP_015252842.1 6-phospho-3-hexuloisomerase -
  DXY22_RS05330 (DXY22_01040) hxlA 1023831..1024463 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  DXY22_RS05335 (DXY22_01041) hxlR 1024695..1025057 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=310304 DXY22_RS05315 WP_003234608.1 1020819..1021268(-) (nucA/comI) [Bacillus subtilis strain SRCM101393]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=310304 DXY22_RS05315 WP_003234608.1 1020819..1021268(-) (nucA/comI) [Bacillus subtilis strain SRCM101393]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment