Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH576_RS03330 Genome accession   NZ_CP031691
Coordinates   663350..663889 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain P615-8618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658350..668889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH576_RS03300 (CH576_03300) fdx 658753..659094 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH576_RS03305 (CH576_03305) iscX 659094..659288 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  CH576_RS03310 (CH576_03310) - 659339..659953 (-) 615 WP_105876738.1 YfgM family protein -
  CH576_RS03315 (CH576_03315) hisS 659971..661242 (-) 1272 WP_116941705.1 histidine--tRNA ligase -
  CH576_RS03320 (CH576_03320) ispG 661252..662358 (-) 1107 WP_005656366.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH576_RS03325 (CH576_03325) - 662367..663278 (-) 912 WP_005662658.1 RodZ family helix-turn-helix domain-containing protein -
  CH576_RS03330 (CH576_03330) pilF/pilF2 663350..663889 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH576_RS03335 (CH576_03335) - 663990..665141 (-) 1152 WP_005669104.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH576_RS03340 (CH576_03340) - 665388..666266 (+) 879 WP_013525570.1 serine hydrolase -
  CH576_RS03345 (CH576_03345) - 666430..667290 (+) 861 WP_223364826.1 metal ABC transporter substrate-binding protein -
  CH576_RS03350 (CH576_03350) - 667290..668210 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=310235 CH576_RS03330 WP_005654843.1 663350..663889(-) (pilF/pilF2) [Haemophilus influenzae strain P615-8618]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=310235 CH576_RS03330 WP_005654843.1 663350..663889(-) (pilF/pilF2) [Haemophilus influenzae strain P615-8618]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTACTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment