Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E3N82_RS01445 Genome accession   NZ_CP038138
Coordinates   311240..312733 (+) Length   497 a.a.
NCBI ID   WP_001040630.1    Uniprot ID   A7ZT00
Organism   Escherichia coli strain G3X16-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 306240..317733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3N82_RS01415 tusA 306487..306732 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  E3N82_RS01420 zntA 306834..309032 (-) 2199 WP_001402600.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  E3N82_RS01425 yhhN 309106..309732 (-) 627 WP_000964718.1 lysoplasmalogenase -
  E3N82_RS01430 yhhM 309873..310232 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  E3N82_RS01435 yhhL 310235..310504 (-) 270 WP_001295207.1 DUF1145 family protein -
  E3N82_RS01440 rsmD 310494..311090 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  E3N82_RS01445 pilA 311240..312733 (+) 1494 WP_001040630.1 signal recognition particle-docking protein FtsY Machinery gene
  E3N82_RS01450 ftsE 312736..313404 (+) 669 WP_086722060.1 cell division ATP-binding protein FtsE -
  E3N82_RS01455 ftsX 313397..314455 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  E3N82_RS01460 rpoH 314700..315554 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  E3N82_RS01465 livJ 315826..316929 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54530.33 Da        Isoelectric Point: 4.1560

>NTDB_id=309527 E3N82_RS01445 WP_001040630.1 311240..312733(+) (pilA) [Escherichia coli strain G3X16-2]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=309527 E3N82_RS01445 WP_001040630.1 311240..312733(+) (pilA) [Escherichia coli strain G3X16-2]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGTAA
CAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGGCGC
GTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.654

81.489

0.388