Detailed information NTG0309372

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: E3U39_RS00255
• Genome accession: NZ_CP038028
• Coordinates: 101459..104044 (-)
• Length: 861 a.a.
• NCBI ID: WP_053573448.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain FS1092
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 96459..109044

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U39_RS00230 (E3U39_00230)	-	96728..96937 (+)	210	WP_015239884.1	hypothetical protein	-
E3U39_RS00235 (E3U39_00235)	-	98162..98464 (-)	303	WP_007409833.1	hypothetical protein	-
E3U39_RS00240 (E3U39_00240)	-	98902..99408 (+)	507	WP_053573450.1	site-specific integrase	-
E3U39_RS00250 (E3U39_00250)	hexB	99569..101443 (-)	1875	WP_053573449.1	DNA mismatch repair endonuclease MutL	Machinery gene
E3U39_RS00255 (E3U39_00255)	hexA	101459..104044 (-)	2586	WP_053573448.1	DNA mismatch repair protein MutS	Machinery gene
E3U39_RS00260 (E3U39_00260)	cotE	104163..104708 (-)	546	WP_003154111.1	outer spore coat protein CotE	-
E3U39_RS00265 (E3U39_00265)	ymcA	104963..105394 (-)	432	WP_003154113.1	RicAFT regulatory complex protein RicA family protein	Regulator
E3U39_RS00270 (E3U39_00270)	miaB	105397..106926 (-)	1530	WP_012117585.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
E3U39_RS00275 (E3U39_00275)	-	107069..108247 (-)	1179	WP_029325912.1	glycine C-acetyltransferase	-

Sequence

Protein

• Length: 861 a.a.
• Molecular weight: 97725.88 Da
• Isoelectric Point: 5.3034

>NTDB_id=309372 E3U39_RS00255 WP_053573448.1 101459..104044(-) (hexA) [Bacillus amyloliquefaciens strain FS1092]
MASYTPMIQQYLKVKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRDGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEDALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQSGDKPELPAVSEKK
SAVREEPAQLSFFTDGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide

• Length: 2586 bp

>NTDB_id=309372 E3U39_RS00255 WP_053573448.1 101459..104044(-) (hexA) [Bacillus amyloliquefaciens strain FS1092]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGGTAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCTTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGATGGAAAAGGCATCCACGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTGACGGATACGGTCTCGCTTTATCGGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCGAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCCACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAAAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCGATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGAGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGGGATTTAATTCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATCAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGATGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAATTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAAGACGGACGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTGAAACAATTTATTCCGCGCTTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTATATGAGAC
AGATCGCACTTCTATCGATCATGGCTCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAGTATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGTCGGGGGATAAACCGGAGCTTCCGGCGGTATCTGAGAAAAAG
TCCGCCGTCAGAGAAGAACCGGCACAGCTGTCCTTTTTTACTGACGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATTAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

• MutS domain II (MutS_II) (125-239)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

• MutS domain I (MutS_I) (5-116)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	48.075	99.535	0.479
mutS	Pseudomonas stutzeri strain ATCC 17587	38.209	100	0.391