Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EKI55_RS01095 Genome accession   NZ_CP037941
Coordinates   210412..211908 (-) Length   498 a.a.
NCBI ID   WP_001040651.1    Uniprot ID   -
Organism   Escherichia coli strain CFSAN027350     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 205412..216908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKI55_RS01070 (EKI55_01070) panM 205636..206019 (+) 384 WP_000778789.1 aspartate 1-decarboxylase autocleavage activator PanM -
  EKI55_RS01075 (EKI55_01075) livJ 206217..207320 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EKI55_RS01080 (EKI55_01080) rpoH 207591..208445 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EKI55_RS01085 (EKI55_01085) ftsX 208690..209748 (-) 1059 WP_001042001.1 permease-like cell division protein FtsX -
  EKI55_RS01090 (EKI55_01090) ftsE 209741..210409 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EKI55_RS01095 (EKI55_01095) pilA 210412..211908 (-) 1497 WP_001040651.1 signal recognition particle-docking protein FtsY Machinery gene
  EKI55_RS01100 (EKI55_01100) rsmD 212058..212654 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EKI55_RS01105 (EKI55_01105) yhhL 212644..212913 (+) 270 WP_001295207.1 DUF1145 family protein -
  EKI55_RS01110 (EKI55_01110) yhhM 212916..213275 (-) 360 WP_000042873.1 DUF2500 domain-containing protein -
  EKI55_RS01115 (EKI55_01115) yhhN 213416..214042 (+) 627 WP_000964718.1 lysoplasmalogenase -
  EKI55_RS01120 (EKI55_01120) zntA 214116..216314 (+) 2199 WP_000106580.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EKI55_RS01125 (EKI55_01125) tusA 216416..216661 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54671.46 Da        Isoelectric Point: 4.1468

>NTDB_id=308933 EKI55_RS01095 WP_001040651.1 210412..211908(-) (pilA) [Escherichia coli strain CFSAN027350]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVAEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=308933 EKI55_RS01095 WP_001040651.1 210412..211908(-) (pilA) [Escherichia coli strain CFSAN027350]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCAGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACTACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGTACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment