Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKAES1M_RS01935 Genome accession   NZ_CP037925
Coordinates   411350..412723 (+) Length   457 a.a.
NCBI ID   WP_019681233.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AES1M     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406350..417723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKAES1M_RS01915 (CKAES1M_00381) - 407187..407753 (-) 567 WP_003084491.1 hypothetical protein -
  CKAES1M_RS01920 (CKAES1M_00382) rsmD 407756..408352 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CKAES1M_RS01925 (CKAES1M_00383) - 408352..409839 (-) 1488 WP_019681235.1 pitrilysin family protein -
  CKAES1M_RS01930 (CKAES1M_00384) - 409832..411229 (-) 1398 WP_019681234.1 pitrilysin family protein -
  CKAES1M_RS01935 (CKAES1M_00385) pilA 411350..412723 (+) 1374 WP_019681233.1 signal recognition particle-docking protein FtsY Machinery gene
  CKAES1M_RS01940 (CKAES1M_00386) ftsE 412720..413391 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  CKAES1M_RS01945 (CKAES1M_00387) ftsX 413391..414398 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  CKAES1M_RS01950 (CKAES1M_00388) rpoH 414512..415366 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  CKAES1M_RS01955 (CKAES1M_00389) - 415431..416036 (+) 606 WP_003084511.1 hypothetical protein -
  CKAES1M_RS01960 (CKAES1M_00390) mtgA 416041..416772 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CKAES1M_RS01965 (CKAES1M_00391) - 416809..417186 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  CKAES1M_RS01970 (CKAES1M_00392) thiS 417289..417489 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48608.01 Da        Isoelectric Point: 4.5033

>NTDB_id=308727 CKAES1M_RS01935 WP_019681233.1 411350..412723(+) (pilA) [Pseudomonas aeruginosa strain AES1M]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAP
VAEAPLAGDEPASAEEHSPRSEAPVAQPEPILAAEPEPEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLS
KTSASIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVE
QPLQVDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSAS
VIFDAVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTG
LALTKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=308727 CKAES1M_RS01935 WP_019681233.1 411350..412723(+) (pilA) [Pseudomonas aeruginosa strain AES1M]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCCGCCG
ATGACGTAGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCG
GTTGCCGAAGCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGTCCGAGGCGCCCGTCGCGCA
ACCCGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTG
CGCCGCTGGCCGCCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCC
AAGACCAGCGCCAGCATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGA
CATCGAGACGCGCCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGG
CGCGCAAGGAGCTGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAG
CAGCCGTTGCAGGTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCAC
CATCGGCAAGCTGGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCTGCCG
CCGTGGAGCAGTTGCAGGTCTGGGGCGAGCGCAACCGTATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCG
GTGATCTTCGACGCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATAC
CAAGGACAACCTGATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCC
TGCTGGTGCTCGACGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGC
CTGGCCCTGACCAAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCG
CTATATCGGTGTCGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGC
GGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(167-232)

SRP54-type protein, GTPase domain (SRP54)

(252-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

46.697

96.061

0.449

Multiple sequence alignment