Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E1J56_RS18820 Genome accession   NZ_CP037910
Coordinates   3830298..3830738 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain YSP8-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3825298..3835738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1J56_RS18800 aroP 3826296..3827669 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  E1J56_RS18805 ampE 3827712..3828566 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  E1J56_RS18810 ampD 3828563..3829114 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E1J56_RS18815 nadC 3829202..3830095 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E1J56_RS18820 pilA 3830298..3830738 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  E1J56_RS18825 pilB 3830748..3832133 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  E1J56_RS18830 hofC 3832123..3833325 (+) 1203 WP_000157266.1 protein transport protein HofC -
  E1J56_RS18835 guaC 3833360..3834403 (-) 1044 WP_001217338.1 GMP reductase -
  E1J56_RS25785 - 3834559..3834603 (-) 45 WP_120795372.1 protein YacM -
  E1J56_RS18840 coaE 3834628..3835248 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=308701 E1J56_RS18820 WP_000360904.1 3830298..3830738(+) (pilA) [Escherichia coli strain YSP8-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=308701 E1J56_RS18820 WP_000360904.1 3830298..3830738(+) (pilA) [Escherichia coli strain YSP8-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363