Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BPHYT_RS02810 Genome accession   NC_010681
Coordinates   629939..631099 (-) Length   386 a.a.
NCBI ID   WP_012431641.1    Uniprot ID   B2SXF8
Organism   Paraburkholderia phytofirmans PsJN     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624939..636099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPHYT_RS02785 (Bphyt_0572) - 625144..625527 (+) 384 WP_012431635.1 nuclear transport factor 2 family protein -
  BPHYT_RS02790 (Bphyt_0573) - 625720..626763 (-) 1044 Protein_566 2-isopropylmalate synthase -
  BPHYT_RS02795 (Bphyt_0575) ybiB 627272..628243 (-) 972 WP_012431638.1 DNA-binding protein YbiB -
  BPHYT_RS02800 (Bphyt_0576) - 628472..629170 (+) 699 WP_012431639.1 fumarylacetoacetate hydrolase family protein -
  BPHYT_RS02805 (Bphyt_0577) maiA 629196..629840 (+) 645 WP_012431640.1 maleylacetoacetate isomerase -
  BPHYT_RS02810 (Bphyt_0578) pilA 629939..631099 (-) 1161 WP_012431641.1 signal recognition particle-docking protein FtsY Machinery gene
  BPHYT_RS02815 (Bphyt_0579) rsmD 631340..631954 (+) 615 WP_012431642.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BPHYT_RS02820 (Bphyt_0580) coaD 632067..632582 (+) 516 WP_012431643.1 pantetheine-phosphate adenylyltransferase -
  BPHYT_RS02825 (Bphyt_0581) - 632646..632903 (+) 258 WP_012431644.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BPHYT_RS02830 (Bphyt_0582) hisC 632981..634060 (-) 1080 WP_012431645.1 histidinol-phosphate transaminase -
  BPHYT_RS02835 (Bphyt_0583) pth 634129..634731 (-) 603 WP_012431646.1 aminoacyl-tRNA hydrolase -
  BPHYT_RS02840 (Bphyt_0584) - 634882..635502 (-) 621 WP_012431647.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 41029.08 Da        Isoelectric Point: 4.8159

>NTDB_id=30853 BPHYT_RS02810 WP_012431641.1 629939..631099(-) (pilA) [Paraburkholderia phytofirmans PsJN]
MFSFFKRFKGSKESDNAPEESQTTPEGSTPTAAVQAPAPVVPAAAPVRVDTPPVAAPSAIDPEPEPEAFALEETVEVVPP
PLPDAGAKRSWLTRLKTGLSKTSSNLTGIFVGTKIDEDLYEELETALLMSDAGVDATEFLLEALREKVRAERLVDPQQVK
TALRTLLVDLLKPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGQRNN
VTVVSQESGDPAAVIFDAVGAARARKIDVMMADTAGRLPTQLHLMEELRKVKRVIGKAQDGAPHEVLLVIDANTGQNALA
QVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPIPVYFIGVGEKVEDLQPFSAEEFSDALLGG

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=30853 BPHYT_RS02810 WP_012431641.1 629939..631099(-) (pilA) [Paraburkholderia phytofirmans PsJN]
ATGTTCAGCTTTTTCAAACGATTCAAGGGTTCGAAAGAGTCCGATAACGCGCCAGAAGAGTCGCAAACCACGCCGGAAGG
TTCGACGCCCACAGCCGCCGTGCAGGCGCCTGCGCCCGTCGTGCCGGCCGCGGCTCCGGTTCGCGTCGACACGCCGCCCG
TGGCCGCTCCGTCGGCAATCGATCCCGAACCCGAGCCTGAAGCATTTGCGCTTGAGGAAACCGTCGAAGTCGTCCCGCCG
CCGTTGCCGGACGCGGGCGCAAAACGTTCGTGGCTGACCCGTCTGAAGACCGGCTTGTCGAAGACGAGTTCGAACCTGAC
CGGCATTTTCGTCGGCACGAAGATTGACGAAGACTTGTACGAAGAGCTCGAAACCGCGCTGCTGATGTCGGACGCGGGCG
TCGACGCCACTGAATTCCTGCTCGAAGCGCTGCGCGAAAAAGTGCGCGCCGAACGCCTCGTCGATCCGCAGCAGGTCAAA
ACCGCGCTGCGCACGTTGCTGGTCGACCTGCTCAAGCCGCTGGAAAAATCGCTCATGCTCGGCCGCGCGCAGCCGCTCGT
GATGATGATTGCCGGCGTCAACGGCGCGGGCAAAACCACCAGCATCGGCAAGCTCGCCAAGCATCTGCAGAGTTTCGATC
AATCCGTGCTGCTGGCCGCGGGCGACACGTTTCGCGCCGCGGCGCGCGAGCAACTGGCCATCTGGGGTCAGCGCAACAAC
GTGACCGTGGTCTCGCAGGAAAGCGGCGACCCGGCAGCGGTAATCTTCGACGCCGTCGGCGCCGCGCGTGCGCGCAAGAT
CGACGTGATGATGGCGGACACGGCCGGCCGCCTGCCGACCCAGTTGCATCTCATGGAAGAACTACGCAAGGTGAAGCGCG
TGATCGGCAAGGCGCAAGACGGCGCGCCGCATGAGGTGCTGCTGGTGATCGACGCCAACACCGGCCAGAACGCGCTCGCG
CAGGTCAAGGCGTTCGACGACGCGCTCGGCCTCACCGGCCTGATCGTCACCAAACTGGACGGCACGGCGAAGGGCGGCAT
TCTCGCCGCGATCGCGCGGCAGCGTCCGATTCCGGTGTATTTCATCGGCGTCGGCGAGAAAGTCGAAGATTTGCAGCCGT
TCAGCGCGGAAGAGTTCTCCGACGCGTTGCTGGGCGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(186-384)

SRP54-type protein, helical bundle domain (SRP54_N)

(105-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2SXF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.11

100

0.575

Multiple sequence alignment