Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E0708_RS07340 Genome accession   NZ_CP037449
Coordinates   1562052..1562729 (+) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain ATCC 25922     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1557052..1567729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0708_RS07305 (E0708_07550) gspG 1557532..1557969 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  E0708_RS07310 (E0708_07555) gspH 1557977..1558486 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  E0708_RS07315 (E0708_07560) gspI 1558483..1558860 (+) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  E0708_RS07320 (E0708_07565) gspJ 1558853..1559440 (+) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  E0708_RS07325 (E0708_07570) gspK 1559433..1560416 (+) 984 Protein_1450 type II secretion system minor pseudopilin GspK -
  E0708_RS07330 (E0708_07575) gspL 1560431..1561594 (+) 1164 WP_001304915.1 type II secretion system protein GspL -
  E0708_RS07335 (E0708_07580) gspM 1561591..1562052 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  E0708_RS07340 (E0708_07585) pilD 1562052..1562729 (+) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  E0708_RS07345 (E0708_07590) bfr 1562758..1563234 (-) 477 WP_000675504.1 bacterioferritin -
  E0708_RS07350 (E0708_07595) bfd 1563306..1563500 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  E0708_RS07355 (E0708_07600) chiA 1563669..1566371 (-) 2703 WP_000773147.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=308420 E0708_RS07340 WP_000178174.1 1562052..1562729(+) (pilD) [Escherichia coli strain ATCC 25922]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=308420 E0708_RS07340 WP_000178174.1 1562052..1562729(+) (pilD) [Escherichia coli strain ATCC 25922]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment