Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EZZ78_RS27090 Genome accession   NZ_CP036492
Coordinates   5858624..5859991 (-) Length   455 a.a.
NCBI ID   WP_003128930.1    Uniprot ID   Q9I6C1
Organism   Pseudomonas aeruginosa strain Paer4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5853624..5864991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ78_RS27055 (EZZ78_27660) thiS 5853858..5854058 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  EZZ78_RS27060 (EZZ78_27665) - 5854161..5854538 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  EZZ78_RS27065 (EZZ78_27670) mtgA 5854575..5855306 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EZZ78_RS27070 (EZZ78_27675) - 5855311..5855916 (-) 606 WP_003084511.1 hypothetical protein -
  EZZ78_RS27075 (EZZ78_27680) rpoH 5855981..5856835 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  EZZ78_RS27080 (EZZ78_27685) ftsX 5856949..5857956 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  EZZ78_RS27085 (EZZ78_27690) ftsE 5857956..5858627 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  EZZ78_RS27090 (EZZ78_27695) pilA 5858624..5859991 (-) 1368 WP_003128930.1 signal recognition particle-docking protein FtsY Machinery gene
  EZZ78_RS27095 (EZZ78_27700) - 5860112..5861509 (+) 1398 WP_010895504.1 M16 family metallopeptidase -
  EZZ78_RS27100 (EZZ78_27705) - 5861502..5862989 (+) 1488 WP_003112971.1 M16 family metallopeptidase -
  EZZ78_RS27105 (EZZ78_27710) rsmD 5862989..5863585 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EZZ78_RS27110 (EZZ78_27715) - 5863588..5864154 (+) 567 WP_003084491.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48391.82 Da        Isoelectric Point: 4.5272

>NTDB_id=308042 EZZ78_RS27090 WP_003128930.1 5858624..5859991(-) (pilA) [Pseudomonas aeruginosa strain Paer4]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAP
VAEAPLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKT
SASIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQP
LQVDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVI
FDAVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLA
LTKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=308042 EZZ78_RS27090 WP_003128930.1 5858624..5859991(-) (pilA) [Pseudomonas aeruginosa strain Paer4]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCG
GTTGCCGAAGCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGCCCCGAGGCGCCCGTCGCACA
ACCCGAGCCTATCCTCGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGC
TGGCCGCCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACC
AGCGCCAGCATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGA
GACGCGCCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCA
AGGAGCTGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCG
TTGCAGGTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGG
CAAGCTGGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGG
AGCAGTTGCAGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATC
TTCGACGCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGA
CAACCTGATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGG
TGCTCGACGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCC
CTGACCAAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATAT
CGGTGTCGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGA
ACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(250-449)

SRP54-type protein, helical bundle domain (SRP54_N)

(165-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9I6C1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

46.911

96.044

0.451