Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BPHY_RS01620 Genome accession   NC_010622
Coordinates   367463..368980 (-) Length   505 a.a.
NCBI ID   WP_012399745.1    Uniprot ID   B2JCQ0
Organism   Paraburkholderia phymatum STM815     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362463..373980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPHY_RS01590 (Bphy_0321) - 363296..363931 (-) 636 WP_012399741.1 LON peptidase substrate-binding domain-containing protein -
  BPHY_RS01595 (Bphy_0322) rapZ 363991..364884 (-) 894 WP_012399742.1 RNase adapter RapZ -
  BPHY_RS01600 (Bphy_0323) hprK 364962..365930 (-) 969 WP_007579699.1 HPr(Ser) kinase/phosphatase -
  BPHY_RS01605 (Bphy_0324) - 366165..366680 (-) 516 WP_012399743.1 PTS sugar transporter subunit IIA -
  BPHY_RS01615 (Bphy_0325) hpf 366930..367289 (-) 360 WP_012399744.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BPHY_RS01620 (Bphy_0326) rpoN/rpoN1 367463..368980 (-) 1518 WP_012399745.1 RNA polymerase factor sigma-54 Machinery gene
  BPHY_RS01625 (Bphy_0327) lptB 369208..369990 (-) 783 WP_012399746.1 LPS export ABC transporter ATP-binding protein -
  BPHY_RS01630 (Bphy_0328) lptA 369987..370664 (-) 678 WP_012399747.1 lipopolysaccharide transport periplasmic protein LptA -
  BPHY_RS01635 (Bphy_0329) lptC 370697..371290 (-) 594 WP_012399748.1 LPS export ABC transporter periplasmic protein LptC -
  BPHY_RS01640 (Bphy_0330) - 371287..371850 (-) 564 WP_012399749.1 HAD family hydrolase -
  BPHY_RS01645 (Bphy_0331) kdsD 371850..372833 (-) 984 WP_041763231.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55447.35 Da        Isoelectric Point: 4.8243

>NTDB_id=30765 BPHY_RS01620 WP_012399745.1 367463..368980(-) (rpoN/rpoN1) [Paraburkholderia phymatum STM815]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAIAQNPLLEAEDDWIASPLRVAADGTVIAQTAQNAAPEEMS
GAPPSSASTSSSESSDNAEPQGVDEYNGLGSDANGDSSQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRVT
QASPRDRALVTFLIESLDDDGYLTATFDEILADLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLCRLDPSP
TRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVRRTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=30765 BPHY_RS01620 WP_012399745.1 367463..368980(-) (rpoN/rpoN1) [Paraburkholderia phymatum STM815]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTTGCGCTGACACCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAACTGCAACAGGAAGTCTCGATGGCGATCGCCCAGAATCCGCTGCTCGAAGCGGAGGACGATTGGA
TCGCGAGCCCGTTGCGCGTCGCCGCTGACGGCACCGTGATCGCGCAAACAGCGCAGAATGCCGCGCCGGAGGAAATGTCC
GGCGCGCCGCCGTCTTCGGCGTCCACGTCTTCGAGCGAAAGCTCGGATAACGCCGAGCCGCAGGGCGTCGACGAGTACAA
CGGGCTGGGTTCGGACGCGAATGGCGATTCGAGCCAGTGGAATCTCGACGACTACGGCCGTTCGGGCAACGCATCGGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGACCACCACGCTGCGCGATCACCTGACTGCTCAACTTCGCGTGACG
CAGGCCAGCCCGCGCGACCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACGGCCACGTT
CGACGAAATACTGGCCGACCTGCCCGAAGAGCTCGAAGTCGATACCGACGAACTGAACGCAGCACTCGCATTGCTGCATA
GCTTCGATCCCGCGGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGCGGCTGCAGCTGTGCCGGCTCGATCCGTCGCCC
ACGCGCACGCTCGCACTCGACATCGTCGCACATCACCTCGAACTGCTCGCCGCACGCGACTTCACGCGTCTGCGCAAGTA
CCTGAAGGCCAGCGACGACGATCTGCGCGACGCCCACGCATTGATCCGCTCGCTCGAACCTTTCCCGGGCGCAGCATACG
GCAAGGCCGAAGCCGACTACGTCGTGCCCGACATCATGGTGCGCAGAACGGCACAGGGGTGGCAGGCAGAACTGAATCCG
GAAGTCGTACCGAAGCTGCGTATCAACCATCTGTACGCGAATATCCTGCGCAACAATCGCGGCGATCCGGGCAGCGGTTC
GCTGCGGCAGCAACTGCAGGAAGCACGCTGGTTGATCAAGAACATCCAGCAACGCTTCGAAACGATTCTGCGCGTGGCAC
AGGCTATTGTGGAGCGTCAAAAGAACTTTTTCGCCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCT
GATACGCTGGGTTTACACGAGTCGACGGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCGCACGTGTCTACCGACACGGGGGGCGCGGCCTCTTCCACGGCCATTCGCGCACTCATCA
AGCAACTGATAGGAGCAGAAGACACCAAATCTCCTCTTTCAGACAGCCGCATTGCCGAACTGCTGGCGGAACAGGGTTTC
GTCGTCGCGCGCCGTACTGTTGCGAAATACCGCGAAGCACTGAAGATCCCGGCAGTCAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2JCQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.525

100

0.675

Multiple sequence alignment