Detailed information NTG0030764

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BPHY_RS01520
• Genome accession: NC_010622
• Coordinates: 350900..352078 (-)
• Length: 392 a.a.
• NCBI ID: WP_012399728.1
• Uniprot ID: B2JCD7
• Organism: Paraburkholderia phymatum STM815
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 345900..357078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BPHY_RS01500 (Bphy_0304)	leuA	346020..347675 (-)	1656	WP_012399724.1	2-isopropylmalate synthase	-
BPHY_RS01505 (Bphy_0305)	ybiB	348195..349163 (-)	969	WP_012399725.1	DNA-binding protein YbiB	-
BPHY_RS01510 (Bphy_0306)	-	349409..350107 (+)	699	WP_012399726.1	fumarylacetoacetate hydrolase family protein	-
BPHY_RS01515 (Bphy_0307)	maiA	350142..350786 (+)	645	WP_012399727.1	maleylacetoacetate isomerase	-
BPHY_RS01520 (Bphy_0308)	pilA	350900..352078 (-)	1179	WP_012399728.1	signal recognition particle-docking protein FtsY	Machinery gene
BPHY_RS01525 (Bphy_0309)	rsmD	352403..353017 (+)	615	WP_012399729.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BPHY_RS01530 (Bphy_0310)	coaD	353135..353641 (+)	507	WP_012399730.1	pantetheine-phosphate adenylyltransferase	-
BPHY_RS01535 (Bphy_0311)	-	353788..354045 (+)	258	WP_012399731.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BPHY_RS01540 (Bphy_0312)	hisC	354115..355206 (-)	1092	WP_012399732.1	histidinol-phosphate transaminase	-
BPHY_RS01545 (Bphy_0313)	pth	355246..355848 (-)	603	WP_012399733.1	aminoacyl-tRNA hydrolase	-
BPHY_RS01550 (Bphy_0314)	-	355969..356580 (-)	612	WP_012399734.1	50S ribosomal protein L25/general stress protein Ctc	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 42201.94 Da
• Isoelectric Point: 4.6426

>NTDB_id=30764 BPHY_RS01520 WP_012399728.1 350900..352078(-) (pilA) [Paraburkholderia phymatum STM815]
MFSFFKRFKGSKAPDDTPEASQTERGADLDMQDLAEPAAPAAARPQALPDSADAPEFDPDYVPDAADFEDADKEADESDA
NVEIVPPPVPEAAARRSWLTRLRSGLSKTSSSITGIFVGTRIDEDLYEELETALLMSDAGVDATEYLLETLREKVRSERL
TDPQQVKSALRTLLVDLLKPLEKSLTLGHYHPTVMMIAGVNGAGKTTSIGKLAKHMQRFNQSVLLAAGDTFRAAAREQLA
IWGERNNVMVVQQESGDPAAVIFDAVGAARARKINVVMADTAGRLPTQLHLMEELRKVKRVIGKAMAGAPHEVLLVIDAN
TGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAEEFSDALLG

Nucleotide

• Length: 1179 bp

>NTDB_id=30764 BPHY_RS01520 WP_012399728.1 350900..352078(-) (pilA) [Paraburkholderia phymatum STM815]
ATGTTCAGCTTTTTCAAACGATTCAAGGGTTCCAAGGCGCCCGACGACACGCCTGAAGCGTCGCAGACCGAGCGCGGCGC
AGACCTCGACATGCAGGACCTGGCTGAGCCGGCAGCGCCCGCGGCGGCCCGCCCGCAAGCTCTGCCGGATTCGGCCGACG
CGCCCGAATTCGATCCAGATTACGTTCCTGATGCCGCCGACTTCGAAGACGCGGACAAGGAAGCCGACGAAAGCGACGCG
AACGTCGAAATTGTTCCGCCGCCCGTGCCCGAAGCGGCCGCCCGACGCTCGTGGCTCACGCGCCTGCGCAGCGGCCTGTC
GAAAACGAGTTCGAGCATCACGGGCATTTTCGTCGGCACCAGGATCGACGAAGACCTGTATGAAGAACTGGAAACGGCGC
TGCTGATGTCCGACGCGGGCGTCGATGCGACCGAATATCTGCTCGAAACGCTGCGCGAGAAAGTACGCAGCGAGCGCCTC
ACCGATCCGCAGCAGGTAAAGAGCGCGCTGCGCACGCTGCTCGTCGACCTGCTGAAGCCGCTGGAGAAGTCGCTTACGCT
CGGCCATTACCATCCGACCGTGATGATGATTGCGGGCGTCAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTGGCCA
AGCATATGCAACGCTTCAACCAGTCCGTGCTGCTCGCAGCGGGTGACACGTTTCGCGCCGCCGCGCGCGAACAGCTCGCG
ATCTGGGGAGAGCGCAATAACGTGATGGTCGTGCAACAGGAAAGCGGCGATCCCGCGGCGGTGATTTTCGATGCCGTCGG
CGCGGCGCGCGCCCGCAAAATCAACGTCGTGATGGCGGACACGGCAGGCCGTCTGCCGACCCAGCTTCACTTGATGGAAG
AACTGCGCAAGGTGAAGCGCGTGATCGGCAAGGCGATGGCCGGCGCGCCGCATGAAGTGCTGCTCGTCATCGATGCGAAT
ACGGGTCAGAATGCGCTCGCGCAAGTAAAGGCATTCGACGACGCACTCGGGCTTACAGGCCTGATCGTGACGAAACTTGA
CGGCACGGCGAAGGGCGGCATCCTCGCGGCGATCGCGCGGCAACGCCCCGTGCCCGTGTACTTCATCGGCGTCGGCGAGA
AGGTCGAGGACCTGCAGCCGTTCAGCGCCGAAGAGTTTTCAGATGCATTGCTGGGTTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (192-391)

• SRP54-type protein, helical bundle domain (SRP54_N) (111-171)

3D structure

• AlphaFold DB: B2JCD7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.257	100	0.561