Detailed information NTG0307322

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: LR49_RS08635
• Genome accession: NZ_CP031537
• Coordinates: 1619684..1621030 (-)
• Length: 448 a.a.
• NCBI ID: WP_000816499.1
• Uniprot ID: Q9R3W9
• Organism: Staphylococcus aureus strain WCH-SK2
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	1599873..1621030	1619684..1621030	within	0

Gene organization within MGE regions

Location: 1599873..1621030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LR49_RS08520 (LR49_08520)	mecA	1599873..1601882 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
LR49_RS08525 (LR49_08525)	mecR1	1601979..1603736 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
LR49_RS08530 (LR49_08530)	-	1603736..1604047 (+)	312	WP_116100490.1	BlaI/MecI/CopY family transcriptional regulator	-
LR49_RS08535 (LR49_08535)	psm-mec	1604192..1604260 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
LR49_RS08540 (LR49_08540)	-	1604594..1605724 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
LR49_RS08550 (LR49_08550)	cstB	1605838..1607172 (-)	1335	Protein_1568	persulfide dioxygenase-sulfurtransferase CstB	-
LR49_RS08555 (LR49_08555)	-	1607205..1608269 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
LR49_RS08560 (LR49_08560)	cstR	1608405..1608665 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
LR49_RS08565 (LR49_08565)	-	1608665..1609303 (+)	639	Protein_1571	sulfite exporter TauE/SafE family protein	-
LR49_RS08575 (LR49_08575)	-	1609577..1610193 (-)	617	Protein_1572	CadD family cadmium resistance transporter	-
LR49_RS08580 (LR49_08580)	-	1610274..1612688 (-)	2415	WP_116100491.1	cadmium-translocating P-type ATPase CadA	-
LR49_RS08585 (LR49_08585)	-	1612681..1613046 (-)	366	WP_000159127.1	ArsR/SmtB family transcription factor	-
LR49_RS08590 (LR49_08590)	-	1613284..1613661 (-)	378	WP_001041908.1	DUF6262 family protein	-
LR49_RS08595 (LR49_08595)	-	1613668..1615560 (-)	1893	WP_002485298.1	site-specific integrase	-
LR49_RS08600 (LR49_08600)	-	1615755..1616078 (-)	324	WP_000088091.1	JAB domain-containing protein	-
LR49_RS08605 (LR49_08605)	-	1616071..1616276 (-)	206	Protein_1578	hypothetical protein	-
LR49_RS08610 (LR49_08610)	-	1616278..1616799 (-)	522	WP_000836008.1	DUF1643 domain-containing protein	-
LR49_RS08615 (LR49_08615)	-	1616818..1617129 (-)	312	WP_000833212.1	DUF960 family protein	-
LR49_RS08625 (LR49_08625)	-	1617214..1617564 (-)	351	WP_000171449.1	SAUGI family uracil-DNA glycosylase inhibitor	-
LR49_RS08630 (LR49_08630)	ccrB	1618035..1619663 (-)	1629	WP_001186596.1	cassette chromosome recombinase CcrB	Machinery gene
LR49_RS08635 (LR49_08635)	ccrA	1619684..1621030 (-)	1347	WP_000816499.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 448 a.a.
• Molecular weight: 52138.19 Da
• Isoelectric Point: 10.1760

>NTDB_id=307322 LR49_RS08635 WP_000816499.1 1619684..1621030(-) (ccrA) [Staphylococcus aureus strain WCH-SK2]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide

• Length: 1347 bp

>NTDB_id=307322 LR49_RS08635 WP_000816499.1 1619684..1621030(-) (ccrA) [Staphylococcus aureus strain WCH-SK2]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (168-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9R3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus COL	78.174	100	0.783
ccrA	Staphylococcus aureus N315	72.383	100	0.725