Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DXT74_RS20815 Genome accession   NZ_CP031514
Coordinates   4558802..4560226 (+) Length   474 a.a.
NCBI ID   WP_194426923.1    Uniprot ID   -
Organism   Chromobacterium sp. Rain0013     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4553802..4565226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXT74_RS20790 (DXT74_20640) - 4555434..4556414 (+) 981 WP_043632493.1 KpsF/GutQ family sugar-phosphate isomerase -
  DXT74_RS20795 (DXT74_20645) - 4556439..4556963 (+) 525 WP_043632491.1 HAD family hydrolase -
  DXT74_RS20800 (DXT74_20650) lptC 4556960..4557529 (+) 570 WP_019104255.1 LPS export ABC transporter periplasmic protein LptC -
  DXT74_RS20805 (DXT74_20655) lptA 4557519..4558073 (+) 555 WP_043591966.1 lipopolysaccharide transport periplasmic protein LptA -
  DXT74_RS20810 (DXT74_20660) lptB 4558070..4558798 (+) 729 WP_019104257.1 LPS export ABC transporter ATP-binding protein -
  DXT74_RS20815 (DXT74_20665) rpoN/rpoN1 4558802..4560226 (+) 1425 WP_194426923.1 RNA polymerase factor sigma-54 Machinery gene
  DXT74_RS20820 (DXT74_20670) hpf 4560294..4560608 (+) 315 WP_043632486.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DXT74_RS20825 (DXT74_20675) ptsN 4560730..4561191 (+) 462 WP_043632481.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DXT74_RS20830 (DXT74_20680) hprK 4561184..4562140 (+) 957 WP_019104261.1 HPr(Ser) kinase/phosphatase -
  DXT74_RS20835 (DXT74_20685) rapZ 4562121..4562963 (+) 843 WP_194426924.1 RNase adapter RapZ -
  DXT74_RS20840 (DXT74_20690) - 4562998..4563633 (+) 636 WP_194426925.1 flavin prenyltransferase UbiX -
  DXT74_RS20845 (DXT74_20695) - 4563679..4565169 (+) 1491 WP_194426926.1 NAD(P)H-hydrate dehydratase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52260.28 Da        Isoelectric Point: 4.7553

>NTDB_id=307260 DXT74_RS20815 WP_194426923.1 4558802..4560226(+) (rpoN/rpoN1) [Chromobacterium sp. Rain0013]
MKQTLQLKVSQQLTLTPQLQQSIKLLQMSTLDLQTEVERFLLDNPLLERGDEAPQGEAADAAAGQRQDGPAETRDEASGD
DGERGAEAGELSDWGSGGRRGDGDDELDPMLNVPCPVSLREHLLAQLGEVALPTRDQAIVQLLIEELDDDGYLGQTLEDI
ASHLPLELEIEADELMIGLRLLQQFDPPGVGARSLPEALALQLQRLDPDEPQRELALAIVRDHLTLLGNRDYTRLKKQLS
VDDAALRAAQALIARLNPRPTAGFGASDTQYVIPDVSVRKRKGRWVAALNGGAVPRLRVNQMYARLLAENRGGNGEMSSR
LQEAKWLVKNIQQRFDTILKVAEAIVERQQSFFEHGEVAMRPLILRDIADELGLHESTVSRVTTQKYLLCPRGLFELKYF
FGSALETDSGGECSATAIKAHIRSLIDAESPSKPLSDSALVEALSKQGIQVARRTIAKYREAMQIPPVNQRKTL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=307260 DXT74_RS20815 WP_194426923.1 4558802..4560226(+) (rpoN/rpoN1) [Chromobacterium sp. Rain0013]
ATGAAACAGACCCTTCAGCTCAAGGTATCGCAGCAGCTGACCCTGACGCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GATGTCCACTCTCGACCTGCAGACCGAAGTCGAGCGCTTCTTGCTGGATAATCCCTTGCTTGAGCGCGGCGACGAAGCGC
CGCAGGGCGAAGCGGCTGACGCCGCCGCCGGCCAGCGACAGGACGGCCCCGCGGAAACCCGCGACGAGGCCTCCGGCGAC
GACGGCGAGCGCGGCGCCGAGGCCGGCGAACTGAGCGACTGGGGCAGCGGCGGCCGCCGCGGCGACGGCGACGACGAACT
GGACCCGATGCTGAACGTACCCTGTCCGGTCAGCCTGCGCGAGCACCTGCTGGCGCAACTGGGCGAAGTGGCGCTGCCCA
CGCGCGACCAGGCCATCGTTCAGTTGCTGATCGAAGAGCTGGACGACGACGGCTACCTAGGTCAAACGCTGGAAGACATC
GCCAGCCACCTGCCGCTGGAACTGGAAATCGAAGCCGACGAACTGATGATAGGCCTGCGCCTGCTGCAACAGTTCGACCC
GCCCGGCGTCGGCGCGCGCTCGCTGCCGGAAGCGCTGGCGCTGCAATTGCAGCGGCTGGATCCGGACGAGCCCCAGCGCG
AGCTGGCGCTGGCCATCGTCCGCGACCACCTGACCTTGCTGGGCAACAGAGACTACACCCGGCTGAAAAAGCAGCTGAGC
GTGGACGACGCCGCGCTGCGCGCCGCCCAGGCGCTGATCGCCCGCCTCAACCCCCGCCCCACCGCCGGTTTCGGCGCCAG
CGACACCCAATACGTGATCCCCGACGTCTCGGTCAGAAAGCGCAAGGGCCGCTGGGTGGCCGCCCTGAACGGCGGCGCCG
TGCCCAGGCTGCGCGTCAACCAGATGTACGCGCGGCTGCTGGCGGAAAACCGCGGCGGCAACGGCGAAATGTCCTCGCGT
CTGCAAGAGGCGAAATGGCTGGTGAAGAACATCCAGCAGCGATTTGACACTATCCTGAAAGTGGCGGAAGCTATCGTTGA
ACGGCAGCAATCGTTCTTTGAACACGGCGAAGTGGCCATGCGCCCGTTAATCCTTCGCGACATCGCGGACGAGCTGGGTT
TGCATGAATCCACGGTTTCCAGAGTGACCACCCAGAAATACCTGCTCTGCCCGCGCGGCCTGTTCGAGCTCAAGTATTTC
TTCGGCAGCGCGCTGGAAACCGACAGCGGCGGCGAGTGCTCCGCCACCGCGATCAAGGCGCATATCCGCAGCCTGATAGA
CGCCGAATCGCCGAGCAAACCGCTGTCCGACAGCGCTCTGGTCGAAGCGCTGTCCAAACAGGGAATACAAGTTGCCAGAC
GCACGATAGCCAAGTATCGTGAAGCCATGCAGATACCGCCGGTCAACCAACGCAAGACGTTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.493

100

0.565

Multiple sequence alignment