Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DS830_RS00810 Genome accession   NZ_CP031513
Coordinates   162852..164231 (+) Length   459 a.a.
NCBI ID   WP_118899404.1    Uniprot ID   -
Organism   Bombilactobacillus bombi strain BI-2.5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 157852..169231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS830_RS00785 (DS830_00785) - 158738..159805 (+) 1068 WP_118907923.1 serine hydrolase domain-containing protein -
  DS830_RS00790 (DS830_00790) - 159875..161218 (-) 1344 WP_118907924.1 C1 family peptidase -
  DS830_RS00795 (DS830_00795) rpiA 161291..161974 (-) 684 WP_118909070.1 ribose-5-phosphate isomerase RpiA -
  DS830_RS00800 (DS830_00800) - 161980..162276 (-) 297 WP_118907925.1 GNAT family N-acetyltransferase -
  DS830_RS00805 (DS830_00805) - 162357..162842 (+) 486 WP_118907926.1 dUTP diphosphatase -
  DS830_RS00810 (DS830_00810) radA 162852..164231 (+) 1380 WP_118899404.1 DNA repair protein RadA Machinery gene
  DS830_RS00815 (DS830_00815) gltX 164302..165792 (+) 1491 WP_118899402.1 glutamate--tRNA ligase -
  DS830_RS00820 (DS830_00820) cysS 165863..167272 (+) 1410 WP_118907927.1 cysteine--tRNA ligase -
  DS830_RS00825 (DS830_00825) - 167269..167682 (+) 414 WP_118899399.1 Mini-ribonuclease 3 -
  DS830_RS00830 (DS830_00830) rlmB 167663..168430 (+) 768 WP_118899397.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DS830_RS00835 (DS830_00835) - 168436..168963 (+) 528 WP_118907928.1 NYN domain-containing protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50173.57 Da        Isoelectric Point: 6.6367

>NTDB_id=307216 DS830_RS00810 WP_118899404.1 162852..164231(+) (radA) [Bombilactobacillus bombi strain BI-2.5]
MAKVKSRYVCQNCGYISPRMLGRCPNCGAWNQLVEELEPTKSTKQNSTSILHHSIAKPQKLTEITFNEEKRVHTGLKELD
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSGRLEQTGGQVLYVSGEESASQIKLRANRLHVGGDNLYIYPETDMSAIEQ
VIDEQKPDYLVIDSVQTMNVPDLSSAIGSVAQIRAVTALLMTIAKQQGISVFVVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHNYRILRSVKNRFGSTNEIGMFAMHNEGLTEVDNPSEVFLEERLANATGSAVVVSMEGTRPILVELQSLITPTLYG
TAKRTTTGLDHNRVSVIMAVLEKRANILLQNQDAYLKATGGVRLDEPAIDLAMAMAIVSSYKNLGLAADECFVGEIGLTG
EIRRVNLIDQRIKEAAKLGFERIYIPANNEVSNDEHYTIEIVGVRTINDAIKKVFKNQH

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=307216 DS830_RS00810 WP_118899404.1 162852..164231(+) (radA) [Bombilactobacillus bombi strain BI-2.5]
GTGGCCAAAGTTAAGTCAAGATATGTATGTCAAAATTGTGGTTATATTTCACCACGAATGTTGGGACGTTGTCCTAATTG
TGGAGCCTGGAATCAACTCGTCGAAGAATTAGAACCAACGAAAAGTACGAAGCAAAACAGTACTAGCATTTTACATCATA
GTATCGCCAAGCCTCAAAAATTAACAGAAATCACTTTTAATGAAGAAAAAAGAGTCCATACAGGGTTGAAAGAACTTGAT
CGTGTACTGGGCGGCGGGGTCGTACCGGGTTCTTTGGTTTTAATTGGTGGTGATCCTGGAATTGGAAAATCAACACTCTT
GCTGCAAGTTTCAGGTAGATTAGAACAAACTGGTGGGCAAGTTTTGTATGTTTCAGGTGAAGAAAGTGCTTCCCAAATTA
AATTGCGGGCTAATCGACTGCATGTTGGCGGCGATAATCTTTATATTTATCCCGAAACTGATATGAGCGCGATTGAACAG
GTCATTGACGAGCAAAAGCCGGATTATTTGGTTATTGATTCGGTGCAAACAATGAACGTTCCTGATTTATCATCAGCAAT
TGGTAGTGTGGCTCAAATTAGAGCAGTTACAGCATTATTGATGACCATTGCGAAACAGCAAGGCATCAGTGTTTTTGTAG
TCGGACATGTGACTAAAGGTGGGGCTATTGCGGGTCCGAAAATTTTAGAACATATGGTCGATACTGTGCTCTATTTTGAA
GGAGATTTGCATCATAATTATCGGATTTTACGTTCTGTTAAAAACCGTTTTGGCTCGACTAATGAAATTGGGATGTTTGC
AATGCACAATGAAGGTTTAACTGAGGTCGATAACCCTTCAGAAGTTTTTTTGGAAGAAAGATTAGCCAACGCAACTGGAT
CAGCGGTAGTGGTTTCGATGGAAGGCACCCGTCCTATCTTAGTAGAATTACAATCATTAATTACACCAACACTTTATGGA
ACAGCCAAACGAACAACAACTGGCTTGGACCATAATCGTGTGTCTGTAATTATGGCTGTTTTGGAAAAGCGGGCTAATAT
CTTATTACAAAATCAAGATGCCTATTTAAAGGCCACTGGTGGGGTCCGTTTGGATGAGCCGGCGATTGATTTGGCAATGG
CAATGGCGATTGTTTCCAGTTATAAGAATCTTGGTTTAGCAGCCGATGAATGTTTTGTGGGAGAAATTGGTTTGACAGGT
GAGATTAGGCGAGTTAATTTGATTGATCAACGCATTAAAGAAGCTGCCAAATTAGGCTTTGAAAGGATTTATATTCCTGC
CAATAACGAAGTTAGTAATGATGAACATTACACAATTGAAATTGTGGGTGTCCGAACGATTAATGATGCAATTAAAAAAG
TTTTTAAAAACCAGCATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-221)

Subunit ChlI of Mg-chelatase (ChlI)

(366-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

62.637

99.129

0.621
  radA Streptococcus mitis NCTC 12261

62.637

99.129

0.621
  radA Streptococcus pneumoniae Rx1

62.637

99.129

0.621
  radA Streptococcus pneumoniae D39

62.637

99.129

0.621
  radA Streptococcus pneumoniae R6

62.637

99.129

0.621
  radA Streptococcus pneumoniae TIGR4

62.637

99.129

0.621
  radA Bacillus subtilis subsp. subtilis str. 168

58.901

99.129

0.584

Multiple sequence alignment