Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DSC91_RS27770 Genome accession   NZ_CP031467
Coordinates   2552028..2553545 (-) Length   505 a.a.
NCBI ID   WP_115781784.1    Uniprot ID   -
Organism   Paraburkholderia caffeinilytica strain CF1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2547028..2558545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSC91_RS27745 (DSC91_005761) - 2547878..2548510 (-) 633 WP_115781781.1 LON peptidase substrate-binding domain-containing protein -
  DSC91_RS27750 (DSC91_005762) rapZ 2548550..2549443 (-) 894 WP_115781782.1 RNase adapter RapZ -
  DSC91_RS27755 (DSC91_005763) hprK 2549562..2550530 (-) 969 WP_012431656.1 HPr(Ser) kinase/phosphatase -
  DSC91_RS27760 (DSC91_005764) - 2550671..2551186 (-) 516 WP_115781783.1 PTS sugar transporter subunit IIA -
  DSC91_RS27765 (DSC91_005765) raiA 2551501..2551857 (-) 357 WP_028199089.1 ribosome-associated translation inhibitor RaiA -
  DSC91_RS27770 (DSC91_005766) rpoN/rpoN1 2552028..2553545 (-) 1518 WP_115781784.1 RNA polymerase factor sigma-54 Machinery gene
  DSC91_RS27775 lptB 2553731..2554516 (-) 786 WP_115781785.1 LPS export ABC transporter ATP-binding protein -
  DSC91_RS27780 (DSC91_005768) lptA 2554513..2555184 (-) 672 WP_115781786.1 lipopolysaccharide transport periplasmic protein LptA -
  DSC91_RS27785 (DSC91_005769) lptC 2555205..2555813 (-) 609 WP_115781787.1 LPS export ABC transporter periplasmic protein LptC -
  DSC91_RS27790 (DSC91_005770) - 2555810..2556370 (-) 561 WP_115781788.1 HAD family hydrolase -
  DSC91_RS27795 (DSC91_005771) kdsD 2556370..2557353 (-) 984 WP_115781789.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55556.68 Da        Isoelectric Point: 5.0733

>NTDB_id=307013 DSC91_RS27770 WP_115781784.1 2552028..2553545(-) (rpoN/rpoN1) [Paraburkholderia caffeinilytica strain CF1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLITQAPNSSAPPDQM
GGNTSSSSTSSSERADNGEPQGVDEYNGLAGDSNGEASQWDLGDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QASQRDRALITFLIESLDDDGYLAATLEEVLTDLPEELEVDLDEMNAALALLHSFDPAGVGARSASECLKLQLLRLEQSP
TRTLALDIVAHHLELLAARDFTRLRKHLKANDDDLRDAHVLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=307013 DSC91_RS27770 WP_115781784.1 2552028..2553545(-) (rpoN/rpoN1) [Paraburkholderia caffeinilytica strain CF1]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCCACGCTCGAACTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAATGAGGACGACTGGA
TCGCGAGTCCCCTGCGCGTGGCGGCCGACGGCTCGCTGATTACCCAGGCGCCCAATTCCTCCGCGCCGCCCGACCAGATG
GGCGGCAATACGTCGTCTTCGTCCACCAGCAGCAGCGAGCGCGCCGACAACGGCGAGCCGCAGGGTGTCGACGAGTACAA
CGGCCTCGCCGGCGACAGCAACGGCGAAGCGTCGCAATGGGATCTGGGCGACTACGGCCGCTCCGGCAACGCCTCGGACG
ACGACGATCTGCCACCGCTGCAAATCCACGAATCGAGCACGTCGCTGCGCGATCACCTGATGGCGCAACTTCGCGTCACC
CAGGCGAGCCAGCGCGACCGCGCGCTAATCACGTTTCTGATCGAATCGCTCGACGACGACGGCTATCTTGCCGCCACGCT
CGAGGAAGTGCTGACCGACCTGCCTGAAGAGCTCGAGGTCGATCTCGACGAAATGAACGCCGCGCTTGCGCTGCTGCACA
GCTTCGACCCGGCAGGGGTCGGCGCACGCTCGGCATCGGAATGTCTGAAGCTGCAATTGCTGCGGCTCGAACAGTCGCCT
ACGCGCACGCTTGCGCTCGACATCGTCGCCCACCACCTGGAACTGCTTGCGGCACGCGATTTCACGCGCCTGCGCAAGCA
CCTGAAGGCGAACGACGACGATCTGCGCGACGCGCATGTGCTGATCCGCTCGCTTGAGCCGTTTCCCGGCGCGGCCTACG
GCAAGGCCGAAGCAGACTACGTCGTGCCGGACATCATGGTGCGCAAAACGGCACAGGGCTGGCAGGCGGAACTCAATCCG
GAAGTGGTGCCGAAGCTGCGCATCAACCATCTGTACGCGAATATTCTGCGCAATAACCGGGGCGATCCTGGGAGCGGATC
GTTGCGCCAGCAACTGCAGGAAGCTCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAGACTATCCTCCGGGTCGCGC
AAGCCATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAATCAACTGTCTCCCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTTTCGACTGACACGGGCGGCGCGGCCTCTTCCACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTTGCGAAGTATCGCGAAGCGCTCAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.211

100

0.671

Multiple sequence alignment