Detailed information NTG0306576

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: EXT04_RS05305
• Genome accession: NZ_CP035944
• Coordinates: 1083253..1084245 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain AR24.2b
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1078253..1089245

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXT04_RS05275 (EXT04_05300)	ispD	1078314..1079024 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
EXT04_RS05280 (EXT04_05305)	ispF	1079024..1079503 (+)	480	WP_001374730.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
EXT04_RS05285 (EXT04_05310)	truD	1079500..1080549 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
EXT04_RS05290 (EXT04_05315)	surE	1080530..1081291 (+)	762	WP_001374723.1	5'/3'-nucleotidase SurE	-
EXT04_RS05295 (EXT04_05320)	pcm	1081285..1081911 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
EXT04_RS05300 (EXT04_05325)	nlpD	1082096..1083190 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
EXT04_RS05305 (EXT04_05330)	rpoS	1083253..1084245 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
EXT04_RS05310 (EXT04_05335)	ygbN	1084339..1085703 (-)	1365	WP_000104456.1	GntP family transporter	-
EXT04_RS05315 (EXT04_05340)	ygbM	1085792..1086568 (-)	777	WP_001136918.1	HPr family phosphocarrier protein	-
EXT04_RS05320 (EXT04_05345)	ygbL	1086573..1087211 (-)	639	WP_001278994.1	aldolase	-
EXT04_RS05325 (EXT04_05350)	otnK	1087208..1088470 (-)	1263	WP_000590392.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=306576 EXT04_RS05305 WP_000081550.1 1083253..1084245(+) (rpoS) [Escherichia coli strain AR24.2b]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=306576 EXT04_RS05305 WP_000081550.1 1083253..1084245(+) (rpoS) [Escherichia coli strain AR24.2b]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715