Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EW995_RS16235 Genome accession   NZ_CP035884
Coordinates   3381942..3382382 (-) Length   146 a.a.
NCBI ID   WP_113444460.1    Uniprot ID   -
Organism   Escherichia coli strain LSN03-016011/A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3376942..3387382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW995_RS16210 coaE 3377432..3378052 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  EW995_RS16215 - 3378077..3378121 (+) 45 WP_120795372.1 protein YacM -
  EW995_RS16220 guaC 3378277..3379320 (+) 1044 WP_001217342.1 GMP reductase -
  EW995_RS16225 hofC 3379355..3380557 (-) 1203 WP_000157227.1 protein transport protein HofC -
  EW995_RS16230 pilB 3380547..3381932 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  EW995_RS16235 pilA 3381942..3382382 (-) 441 WP_113444460.1 prepilin peptidase-dependent pilin Machinery gene
  EW995_RS16240 nadC 3382585..3383478 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EW995_RS16245 ampD 3383566..3384117 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EW995_RS16250 ampE 3384114..3384968 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EW995_RS16255 aroP 3385011..3386384 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.3927

>NTDB_id=306490 EW995_RS16235 WP_113444460.1 3381942..3382382(-) (pilA) [Escherichia coli strain LSN03-016011/A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQVCEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306490 EW995_RS16235 WP_113444460.1 3381942..3382382(-) (pilA) [Escherichia coli strain LSN03-016011/A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGTCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment