Detailed information NTG0306446

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: EW995_RS06440
• Genome accession: NZ_CP035884
• Coordinates: 1330687..1331679 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain LSN03-016011/A
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1325687..1336679

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW995_RS06420	otnK	1326462..1327724 (+)	1263	WP_000590392.1	3-oxo-tetronate kinase	-
EW995_RS06425	ygbL	1327721..1328359 (+)	639	WP_001278994.1	aldolase	-
EW995_RS06430	ygbM	1328364..1329140 (+)	777	WP_001136918.1	HPr family phosphocarrier protein	-
EW995_RS06435	ygbN	1329229..1330593 (+)	1365	WP_000104456.1	GntP family transporter	-
EW995_RS06440	rpoS	1330687..1331679 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
EW995_RS06445	nlpD	1331742..1332836 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
EW995_RS06450	pcm	1333021..1333647 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
EW995_RS06455	surE	1333641..1334402 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
EW995_RS06460	truD	1334383..1335432 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
EW995_RS06465	ispF	1335429..1335908 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
EW995_RS06470	ispD	1335908..1336618 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=306446 EW995_RS06440 WP_000081550.1 1330687..1331679(-) (rpoS) [Escherichia coli strain LSN03-016011/A]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=306446 EW995_RS06440 WP_000081550.1 1330687..1331679(-) (rpoS) [Escherichia coli strain LSN03-016011/A]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715