Detailed information NTG0306329

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: EW997_RS20460
• Genome accession: NZ_CP035879
• Coordinates: 4146324..4147316 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain DS26-1
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 4141324..4152316

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW997_RS20430	ispD	4141385..4142095 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
EW997_RS20435	ispF	4142095..4142574 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
EW997_RS20440	truD	4142571..4143620 (+)	1050	WP_074506867.1	tRNA pseudouridine(13) synthase TruD	-
EW997_RS20445	surE	4143601..4144362 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
EW997_RS20450	pcm	4144356..4144982 (+)	627	WP_000254703.1	protein-L-isoaspartate O-methyltransferase	-
EW997_RS20455	nlpD	4145167..4146261 (+)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
EW997_RS20460	rpoS	4146324..4147316 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
EW997_RS20465	-	4147437..4147844 (-)	408	WP_001208070.1	MarR family winged helix-turn-helix transcriptional regulator	-
EW997_RS20470	-	4147991..4148584 (+)	594	WP_000767718.1	non-oxidative hydroxyarylic acid decarboxylases subunit B	-
EW997_RS20475	-	4148584..4150011 (+)	1428	WP_000863195.1	non-oxidative hydroxyarylic acid decarboxylases subunit C	-
EW997_RS20480	-	4150022..4150258 (+)	237	WP_000562984.1	non-oxidative hydroxyarylic acid decarboxylases subunit D	-
EW997_RS20485	ygbN	4150297..4151661 (-)	1365	WP_001363538.1	GntP family transporter	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=306329 EW997_RS20460 WP_000081550.1 4146324..4147316(+) (rpoS) [Escherichia coli strain DS26-1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=306329 EW997_RS20460 WP_000081550.1 4146324..4147316(+) (rpoS) [Escherichia coli strain DS26-1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGATGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715