Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EW997_RS15655 Genome accession   NZ_CP035879
Coordinates   3155265..3155933 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain DS26-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3150265..3160933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW997_RS15630 pyrE 3150790..3151431 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EW997_RS15635 - 3151729..3152688 (+) 960 WP_000340183.1 reverse transcriptase family protein -
  EW997_RS15640 slmA 3152734..3153330 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EW997_RS15645 dut 3153437..3153895 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EW997_RS15650 coaBC 3153873..3155093 (-) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EW997_RS15655 radC 3155265..3155933 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EW997_RS15660 rpmB 3156150..3156386 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EW997_RS15665 rpmG 3156407..3156574 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EW997_RS15670 mutM 3156672..3157481 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EW997_RS15675 coaD 3157520..3157999 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EW997_RS15680 waaA 3158007..3159284 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EW997_RS15685 rfaQ 3159697..3160755 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=306313 EW997_RS15655 WP_001297375.1 3155265..3155933(+) (radC) [Escherichia coli strain DS26-1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=306313 EW997_RS15655 WP_001297375.1 3155265..3155933(+) (radC) [Escherichia coli strain DS26-1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment