Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EW998_RS10445 Genome accession   NZ_CP035874
Coordinates   2202597..2203073 (-) Length   158 a.a.
NCBI ID   WP_001186773.1    Uniprot ID   -
Organism   Escherichia coli strain B2C     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2197597..2208073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW998_RS10395 sbmC 2198090..2198563 (+) 474 WP_001394439.1 DNA gyrase inhibitor SbmC -
  EW998_RS10400 yeeA 2198761..2199819 (+) 1059 WP_001200905.1 FUSC family protein -
  EW998_RS10405 tmaR 2199991..2200320 (+) 330 WP_000450409.1 DUF496 family protein -
  EW998_RS10410 - 2200421..2200603 (-) 183 WP_024169664.1 EutP/PduV family microcompartment system protein -
  EW998_RS10415 - 2200657..2200803 (+) 147 Protein_2034 transposase domain-containing protein -
  EW998_RS10420 - 2201092..2201172 (-) 81 Protein_2035 hypothetical protein -
  EW998_RS10425 yeeW 2201218..2201412 (-) 195 WP_000988599.1 DUF5983 family protein -
  EW998_RS10430 cbtA 2201409..2201782 (-) 374 Protein_2037 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA -
  EW998_RS10435 cbeA 2201871..2202239 (-) 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA -
  EW998_RS10440 yeeT 2202313..2202534 (-) 222 WP_000692323.1 DUF987 domain-containing protein -
  EW998_RS10445 radC 2202597..2203073 (-) 477 WP_001186773.1 RadC family protein Machinery gene
  EW998_RS10450 - 2203089..2203568 (-) 480 WP_000860076.1 antirestriction protein -
  EW998_RS10455 - 2203650..2204471 (-) 822 WP_001234530.1 DUF932 domain-containing protein -
  EW998_RS25860 - 2204692..2204769 (-) 78 Protein_2043 hypothetical protein -
  EW998_RS25865 - 2204761..2204949 (+) 189 Protein_2044 IS4 family transposase -
  EW998_RS25870 - 2204946..2205119 (+) 174 Protein_2045 cobalamin biosynthesis protein -
  EW998_RS10460 cobU 2205253..2205799 (+) 547 Protein_2046 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  EW998_RS10465 cobS 2205796..2206539 (+) 744 WP_001355648.1 adenosylcobinamide-GDP ribazoletransferase -
  EW998_RS10470 cobT 2206551..2207630 (+) 1080 WP_001193822.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 17688.29 Da        Isoelectric Point: 7.2020

>NTDB_id=306236 EW998_RS10445 WP_001186773.1 2202597..2203073(-) (radC) [Escherichia coli strain B2C]
MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGT
INRTEVHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=306236 EW998_RS10445 WP_001186773.1 2202597..2203073(-) (radC) [Escherichia coli strain B2C]
ATGCAACAACTTTCCTTTCTGCCCGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGA
CCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAC
TGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAATAACCAGAATCAACTGATTGCCGGTGAAACCCTCTTTACCGGCACC
ATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGTGGTGCTGGCACA
CAATCACCCCTCCGGTGAAGTCACACCCAGTAAGGCAGACCGCCTTATCACGGAACGTCTGGTACAGGCACTGGGCCTGG
TGGATATCCGGGTGCCGGACCATCTGATAGTCGGTGGCAACCAGGTTTTCTCCTTTGCCGAACATGGTCTGCTTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(38-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

54.867

71.519

0.392

Multiple sequence alignment