Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   EW998_RS06960 Genome accession   NZ_CP035874
Coordinates   1452360..1453352 (+) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain B2C     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 1447360..1458352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW998_RS06930 ispD 1447421..1448131 (+) 711 WP_000246135.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EW998_RS06935 ispF 1448131..1448610 (+) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EW998_RS06940 truD 1448607..1449656 (+) 1050 WP_000568946.1 tRNA pseudouridine(13) synthase TruD -
  EW998_RS06945 surE 1449637..1450398 (+) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  EW998_RS06950 pcm 1450392..1451018 (+) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  EW998_RS06955 nlpD 1451203..1452297 (+) 1095 WP_224692580.1 murein hydrolase activator NlpD -
  EW998_RS06960 rpoS 1452360..1453352 (+) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  EW998_RS06965 ygbN 1453446..1454808 (-) 1363 Protein_1361 GntP family transporter -
  EW998_RS06970 ygbM 1454897..1455673 (-) 777 WP_001136934.1 HPr family phosphocarrier protein -
  EW998_RS06975 ygbL 1455678..1456316 (-) 639 WP_001278994.1 aldolase -
  EW998_RS06980 otnK 1456313..1457575 (-) 1263 WP_000590403.1 3-oxo-tetronate kinase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=306222 EW998_RS06960 WP_000081550.1 1452360..1453352(+) (rpoS) [Escherichia coli strain B2C]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=306222 EW998_RS06960 WP_000081550.1 1452360..1453352(+) (rpoS) [Escherichia coli strain B2C]
CTGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715