Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EW998_RS02295 Genome accession   NZ_CP035874
Coordinates   487238..487906 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain B2C     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 482238..492906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW998_RS02265 yicC 482257..483120 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  EW998_RS02270 rph 483247..483963 (+) 717 WP_001247089.1 ribonuclease PH -
  EW998_RS02275 pyrE 484029..484670 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EW998_RS02280 slmA 484707..485303 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EW998_RS02285 dut 485410..485868 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EW998_RS02290 coaBC 485846..487066 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EW998_RS02295 radC 487238..487906 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EW998_RS02300 rpmB 488123..488359 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EW998_RS02305 rpmG 488380..488547 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EW998_RS02310 mutM 488645..489454 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EW998_RS02315 coaD 489493..489972 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EW998_RS02320 waaA 489980..491257 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EW998_RS02325 rfaQ 491670..492728 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=306204 EW998_RS02295 WP_001297375.1 487238..487906(+) (radC) [Escherichia coli strain B2C]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=306204 EW998_RS02295 WP_001297375.1 487238..487906(+) (radC) [Escherichia coli strain B2C]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496