Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   EW999_RS21260 Genome accession   NZ_CP035870
Coordinates   4349166..4350158 (+) Length   330 a.a.
NCBI ID   WP_000081575.1    Uniprot ID   -
Organism   Escherichia coli strain E2528-C1     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 4344166..4355158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS21230 ispD 4344227..4344937 (+) 711 WP_000246135.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EW999_RS21235 ispF 4344937..4345416 (+) 480 WP_001735626.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EW999_RS21240 truD 4345413..4346462 (+) 1050 WP_000568946.1 tRNA pseudouridine(13) synthase TruD -
  EW999_RS21245 surE 4346443..4347204 (+) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  EW999_RS21250 pcm 4347198..4347824 (+) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  EW999_RS21255 nlpD 4348009..4349103 (+) 1095 WP_224692580.1 murein hydrolase activator NlpD -
  EW999_RS21260 rpoS 4349166..4350158 (+) 993 WP_000081575.1 RNA polymerase sigma factor RpoS Regulator
  EW999_RS21265 ygbN 4350252..4351616 (-) 1365 WP_001735624.1 GntP family transporter -
  EW999_RS21270 ygbM 4351705..4352481 (-) 777 WP_001136934.1 HPr family phosphocarrier protein -
  EW999_RS21275 - 4352486..4353055 (-) 570 Protein_4196 aldolase -
  EW999_RS21285 - 4353824..4353955 (-) 132 Protein_4198 serine/threonine protein phosphatase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37946.76 Da        Isoelectric Point: 4.5597

>NTDB_id=306176 EW999_RS21260 WP_000081575.1 4349166..4350158(+) (rpoS) [Escherichia coli strain E2528-C1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLSRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=306176 EW999_RS21260 WP_000081575.1 4349166..4350158(+) (rpoS) [Escherichia coli strain E2528-C1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGAGCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

72.755

97.879

0.712