Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EW999_RS17410 Genome accession   NZ_CP035870
Coordinates   3555188..3556684 (+) Length   498 a.a.
NCBI ID   WP_032199781.1    Uniprot ID   -
Organism   Escherichia coli strain E2528-C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3550188..3561684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS17380 tusA 3550435..3550680 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  EW999_RS17385 zntA 3550782..3552980 (-) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EW999_RS17390 yhhN 3553054..3553680 (-) 627 WP_000964718.1 lysoplasmalogenase -
  EW999_RS17395 yhhM 3553821..3554180 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  EW999_RS17400 yhhL 3554183..3554452 (-) 270 WP_001311191.1 DUF1145 family protein -
  EW999_RS17405 rsmD 3554442..3555038 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EW999_RS17410 pilA 3555188..3556684 (+) 1497 WP_032199781.1 signal recognition particle-docking protein FtsY Machinery gene
  EW999_RS17415 ftsE 3556687..3557355 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EW999_RS17420 ftsX 3557348..3558406 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  EW999_RS17425 rpoH 3558651..3559505 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EW999_RS17430 livJ 3559776..3560879 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EW999_RS17435 panM 3561079..3561462 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54698.57 Da        Isoelectric Point: 4.1734

>NTDB_id=306158 EW999_RS17410 WP_032199781.1 3555188..3556684(+) (pilA) [Escherichia coli strain E2528-C1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDKELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=306158 EW999_RS17410 WP_032199781.1 3555188..3556684(+) (pilA) [Escherichia coli strain E2528-C1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAACCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACAAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment