Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EW999_RS16430 Genome accession   NZ_CP035870
Coordinates   3335802..3336470 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain E2528-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3330802..3341470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS16400 yicC 3330821..3331684 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  EW999_RS16405 rph 3331811..3332527 (+) 717 WP_001247089.1 ribonuclease PH -
  EW999_RS16410 pyrE 3332593..3333234 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EW999_RS16415 slmA 3333271..3333867 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EW999_RS16420 dut 3333974..3334432 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EW999_RS16425 coaBC 3334410..3335630 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EW999_RS16430 radC 3335802..3336470 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EW999_RS16435 rpmB 3336687..3336923 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EW999_RS16440 rpmG 3336944..3337111 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EW999_RS16445 mutM 3337209..3338018 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EW999_RS16450 coaD 3338057..3338536 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EW999_RS16455 waaA 3338544..3339821 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EW999_RS16460 rfaQ 3340234..3341292 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=306155 EW999_RS16430 WP_001297375.1 3335802..3336470(+) (radC) [Escherichia coli strain E2528-C1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=306155 EW999_RS16430 WP_001297375.1 3335802..3336470(+) (radC) [Escherichia coli strain E2528-C1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496