Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   EW999_RS15500 Genome accession   NZ_CP035870
Coordinates   3143024..3144853 (-) Length   609 a.a.
NCBI ID   WP_001395096.1    Uniprot ID   -
Organism   Escherichia coli strain E2528-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3138024..3149853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS15475 bioP 3138742..3139641 (-) 900 WP_001196237.1 biotin transporter -
  EW999_RS15480 yigL 3139717..3140517 (-) 801 WP_000285362.1 sugar/pyridoxal phosphate phosphatase YigL -
  EW999_RS15485 pldB 3140525..3141547 (-) 1023 WP_000487640.1 lysophospholipase L2 -
  EW999_RS15490 rhtB 3141658..3142278 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  EW999_RS15495 rhtC 3142340..3142960 (-) 621 WP_000928821.1 threonine export protein RhtC -
  EW999_RS15500 recQ 3143024..3144853 (-) 1830 WP_001395096.1 ATP-dependent DNA helicase RecQ Machinery gene
  EW999_RS15505 pldA 3144986..3145855 (-) 870 WP_001735928.1 phospholipase A -
  EW999_RS15510 yigI 3146020..3146481 (+) 462 WP_004987787.1 acyl-CoA thioesterase YigI -
  EW999_RS15515 rarD 3146533..3147423 (+) 891 WP_000339104.1 EamA family transporter RarD -
  EW999_RS15520 yigG 3147518..3147898 (+) 381 WP_000944218.1 protein YigG -
  EW999_RS15525 yigF 3147912..3148292 (+) 381 WP_000032581.1 DUF2628 domain-containing protein -
  EW999_RS15530 corA 3148335..3149285 (-) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  EW999_RS15535 ysgD 3149437..3149493 (-) 57 WP_211180520.1 protein YsgD -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68373.55 Da        Isoelectric Point: 7.2544

>NTDB_id=306145 EW999_RS15500 WP_001395096.1 3143024..3144853(-) (recQ) [Escherichia coli strain E2528-C1]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKPGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=306145 EW999_RS15500 WP_001395096.1 3143024..3144853(-) (recQ) [Escherichia coli strain E2528-C1]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
GATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGCCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCTGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTTATCCGCGCGCATGTTGACGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment