Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EW999_RS12015 Genome accession   NZ_CP035870
Coordinates   2400403..2400843 (+) Length   146 a.a.
NCBI ID   WP_001727303.1    Uniprot ID   -
Organism   Escherichia coli strain E2528-C1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395403..2405843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS11995 aroP 2396401..2397774 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  EW999_RS12000 ampE 2397817..2398671 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  EW999_RS12005 ampD 2398668..2399219 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EW999_RS12010 nadC 2399307..2400200 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EW999_RS12015 pilA 2400403..2400843 (+) 441 WP_001727303.1 prepilin peptidase-dependent pilin Machinery gene
  EW999_RS12020 pilB 2400853..2402238 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  EW999_RS12025 hofC 2402228..2403430 (+) 1203 WP_021566125.1 protein transport protein HofC -
  EW999_RS12030 guaC 2403465..2404508 (-) 1044 WP_001217338.1 GMP reductase -
  EW999_RS12035 - 2404664..2404708 (-) 45 WP_120795372.1 protein YacM -
  EW999_RS12040 coaE 2404733..2405353 (+) 621 WP_001269532.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15649.81 Da        Isoelectric Point: 4.3938

>NTDB_id=306131 EW999_RS12015 WP_001727303.1 2400403..2400843(+) (pilA) [Escherichia coli strain E2528-C1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVVKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306131 EW999_RS12015 WP_001727303.1 2400403..2400843(+) (pilA) [Escherichia coli strain E2528-C1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGTAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363