Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EW999_RS01235 Genome accession   NZ_CP035870
Coordinates   263817..265244 (-) Length   475 a.a.
NCBI ID   WP_000980570.1    Uniprot ID   -
Organism   Escherichia coli strain E2528-C1     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 258817..270244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW999_RS01205 yeeZ 258872..259696 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  EW999_RS01210 yeeY 259742..260671 (+) 930 WP_000803337.1 LysR substrate-binding domain-containing protein -
  EW999_RS01215 yoeI 260886..260948 (+) 63 WP_010723108.1 membrane protein YoeI -
  EW999_RS01220 plaP 260938..262296 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  EW999_RS01225 tsuA 262475..263533 (+) 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
  EW999_RS01230 tsuB 263547..263774 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EW999_RS01235 sbcB 263817..265244 (-) 1428 WP_000980570.1 exodeoxyribonuclease I Machinery gene
  EW999_RS01240 dacD 265453..266619 (+) 1167 WP_001735394.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EW999_RS01245 sbmC 266738..267211 (+) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  EW999_RS01250 yeeA 267409..268467 (+) 1059 WP_001200905.1 FUSC family protein -
  EW999_RS01255 tmaR 268639..268968 (+) 330 WP_000450409.1 DUF496 family protein -
  EW999_RS25950 - 269069..269251 (-) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  EW999_RS25955 - 269371..269451 (+) 81 WP_023441679.1 hypothetical protein -
  EW999_RS01270 - 269740..269820 (-) 81 Protein_254 hypothetical protein -
  EW999_RS01275 yeeW 269866..270060 (-) 195 WP_000988599.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54529.81 Da        Isoelectric Point: 5.4720

>NTDB_id=306106 EW999_RS01235 WP_000980570.1 263817..265244(-) (sbcB) [Escherichia coli strain E2528-C1]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=306106 EW999_RS01235 WP_000980570.1 263817..265244(-) (sbcB) [Escherichia coli strain E2528-C1]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCAGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGCGCAGCAATGAAAATTGTGCTGGAAACCGAGCCACGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581