Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXE34_RS02460 Genome accession   NZ_CP035865
Coordinates   515076..515516 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain E275 a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 510076..520516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXE34_RS02435 coaE 510566..511186 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  EXE34_RS02440 - 511211..511255 (+) 45 WP_120795372.1 protein YacM -
  EXE34_RS02445 guaC 511411..512454 (+) 1044 WP_001217337.1 GMP reductase -
  EXE34_RS02450 hofC 512489..513691 (-) 1203 WP_000157266.1 protein transport protein HofC -
  EXE34_RS02455 pilB 513681..515066 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  EXE34_RS02460 pilA 515076..515516 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  EXE34_RS02465 nadC 515719..516612 (-) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXE34_RS02470 ampD 516700..517251 (+) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXE34_RS02475 ampE 517248..518102 (+) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  EXE34_RS02480 aroP 518145..519518 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=306028 EXE34_RS02460 WP_000360904.1 515076..515516(-) (pilA) [Escherichia coli strain E275 a]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306028 EXE34_RS02460 WP_000360904.1 515076..515516(-) (pilA) [Escherichia coli strain E275 a]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment