Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA00_RS19725 Genome accession   NZ_CP035860
Coordinates   4006628..4007068 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain TD225-C4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4001628..4012068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA00_RS19705 aroP 4002626..4003999 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EXA00_RS19710 ampE 4004042..4004896 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EXA00_RS19715 ampD 4004893..4005444 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXA00_RS19720 nadC 4005532..4006425 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXA00_RS19725 pilA 4006628..4007068 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  EXA00_RS19730 pilB 4007078..4008463 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  EXA00_RS19735 hofC 4008453..4009655 (+) 1203 WP_000157227.1 protein transport protein HofC -
  EXA00_RS19740 guaC 4009690..4010733 (-) 1044 WP_001217342.1 GMP reductase -
  EXA00_RS19745 - 4010889..4010933 (-) 45 WP_120795372.1 protein YacM -
  EXA00_RS19750 coaE 4010958..4011578 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=306001 EXA00_RS19725 WP_000360895.1 4006628..4007068(+) (pilA) [Escherichia coli strain TD225-C4]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306001 EXA00_RS19725 WP_000360895.1 4006628..4007068(+) (pilA) [Escherichia coli strain TD225-C4]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment