Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EXA00_RS00700 Genome accession   NZ_CP035860
Coordinates   144154..144822 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain TD225-C4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 139154..149822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA00_RS00675 pyrE 139679..140320 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EXA00_RS00680 - 140618..141577 (+) 960 WP_000340183.1 reverse transcriptase family protein -
  EXA00_RS00685 slmA 141623..142219 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EXA00_RS00690 dut 142326..142784 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EXA00_RS00695 coaBC 142762..143982 (-) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EXA00_RS00700 radC 144154..144822 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EXA00_RS00705 rpmB 145039..145275 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EXA00_RS00710 rpmG 145296..145463 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EXA00_RS00715 mutM 145561..146370 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EXA00_RS00720 coaD 146409..146888 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EXA00_RS00725 waaA 146896..148173 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EXA00_RS00730 rfaQ 148586..149644 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=305946 EXA00_RS00700 WP_001297375.1 144154..144822(+) (radC) [Escherichia coli strain TD225-C4]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=305946 EXA00_RS00700 WP_001297375.1 144154..144822(+) (radC) [Escherichia coli strain TD225-C4]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment