Detailed information    

experimental Experimentally validated

Overview

Name   tpc   Type   Machinery gene
Locus tag   NGFG_RS01435 Genome accession   NC_022240
Coordinates   264191..265231 (-) Length   346 a.a.
NCBI ID   WP_003687618.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   DNA processing; DNA transport into the cytoplasm   
DNA processing DNA binding and uptake

Function

Three competence factors unrelated to pilus biogenesis, ComA, ComL and Tpc, are not essential for DNA uptake and rather act in a subsequent step. The respective mutants, however, lack the characteristic nucleolytic processing observed with the incoming DNA in both wild-type and non-transformable RecA-deficient N. gonorrhoeae, indicating that they are blocked in the processing and/or the delivery of DNA to the cytoplasm.


Genomic Context

Location: 259191..270231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS01415 (NGFG_00401) - 260687..261313 (-) 627 WP_003687611.1 phosphoribosylanthranilate isomerase -
  NGFG_RS01420 (NGFG_00402) greB 261372..261863 (-) 492 WP_003687613.1 transcription elongation factor GreB -
  NGFG_RS01425 (NGFG_00403) purF 261963..263507 (-) 1545 WP_003694871.1 amidophosphoribosyltransferase -
  NGFG_RS01430 (NGFG_00405) - 263701..264198 (-) 498 WP_003687616.1 CvpA family protein -
  NGFG_RS01435 (NGFG_00406) tpc 264191..265231 (-) 1041 WP_003687618.1 cell division protein FtsN Machinery gene
  NGFG_RS01440 (NGFG_00407) folC 265245..266519 (-) 1275 WP_003687619.1 bifunctional tetrahydrofolate synthase/dihydrofolate synthase -
  NGFG_RS01445 (NGFG_00408) - 266550..266990 (-) 441 WP_003687622.1 protein YgfX -
  NGFG_RS01450 (NGFG_00409) - 266995..267354 (-) 360 WP_003687624.1 hypothetical protein -
  NGFG_RS01455 (NGFG_00410) - 267427..268155 (-) 729 WP_010357138.1 amino acid ABC transporter ATP-binding protein -
  NGFG_RS01460 (NGFG_00411) - 268197..268652 (-) 456 WP_003690747.1 hypothetical protein -
  NGFG_RS01465 (NGFG_00412) - 268763..269332 (-) 570 WP_003687630.1 GTP pyrophosphokinase -
  NGFG_RS01470 (NGFG_00413) rsmA 269347..270126 (-) 780 WP_003687633.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 36992.15 Da        Isoelectric Point: 9.8118

>NTDB_id=1129 NGFG_RS01435 WP_003687618.1 264191..265231(-) (tpc) [Neisseria gonorrhoeae MS11]
MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSSDPADSNPAPQAGETGATESQTANTAQTPALKSAAEN
GETAADKPQDLAGEDKPSAADSEISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
DSTDTVAVEKPKRSAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKADKAEGKKTAEK
DRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAAIQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSN
YKNARDAERDLNKLRVHGIAGQVTNE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1129 NGFG_RS01435 WP_003687618.1 264191..265231(-) (tpc) [Neisseria gonorrhoeae MS11]
ATGTCCGAAAACAAACAAAACGAAGTCCTGACCGGTTACGAACAGCTGAAACGGCGCAACCGCCGCCGCCTCGTAACGGC
AAGCTCCCTGGTTGCCGCCTCCTGCATCCTGCTGGCAGCCGCACTCAGTTCCGATCCTGCCGACAGCAATCCCGCACCGC
AGGCCGGCGAAACCGGCGCAACGGAAAGCCAAACGGCAAACACGGCACAAACCCCTGCCTTGAAATCCGCCGCCGAAAAC
GGGGAAACCGCCGCCGACAAACCGCAGGACTTGGCAGGCGAAGACAAGCCTTCTGCCGCCGACAGCGAAATCAGCGAGCC
TGAAAACGTAGGCGCGCCGCTGGTGCTGATTAACGACCGGCTCGAAGACAGCAACATCAAAGGTTTGGAAGAATCCGAGA
AACTGCAACAGGCAGAAACCGCCAAAACCGAACCGAAGCAGGCAAAACAACGCGCTGCCGAAAAAGTGTCGGCAACTGCC
GACAGTACGGATACGGTAGCGGTTGAAAAACCGAAACGCTCTGCCGAACCCAAACCGCAAAAAGCGGAACGCACTGCCGA
AGCCAAGCCCAAAGCCAAAGAAACCAAAACCGCCGAAAAAGTTGCCGACAAACCGAAAACTGCTGCCGAAAAAACCAAAC
CGGATACGGCAAAATCCGACAGCGCGGTAAAAGAAGCGAAAAAAGCCGACAAGGCTGAAGGCAAAAAGACAGCCGAAAAA
GACCGTTCGGACGGCAAAAAACACGAAACGGCGCAAAAAACCGACAAAGCGGACAAAACCAAAACCGCCGAGAAGGAAAA
ATCCGGCAAGGCGGGCAAAAAAGCCGCCATTCAGGCAGGTTATGCCGAAAAAGAACGCGCCTTGAGCCTCCAGCGCAAAA
TGAAGGCGGCGGGTATCGATTCGACCATCACCGAAATCATGACCGACAACGGCAAAGTTTACCGCGTCAAATCAAGCAAC
TATAAAAACGCAAGGGATGCCGAACGCGATTTGAACAAACTGCGCGTGCACGGCATCGCCGGCCAGGTAACGAATGAATA
G

Domains

Predicted by InterproScan.

SPOR domain (SPOR)

(272-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] M Fussenegger et al. (1996) Tetrapac (tpc), a novel genotype of Neisseria gonorrhoeae affecting epithelial cell invasion, natural transformation competence and cell separation. Molecular Microbiology 19(6):1357-72. [PMID: 8730876]
[2] D Facius et al. (1996) Sequential action of factors involved in natural competence for transformation of Neisseria gonorrhoeae. FEMS Microbiology Letters 137(2-3):159-64. [PMID: 8998979]