Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA01_RS14515 Genome accession   NZ_CP035846
Coordinates   3010873..3012369 (+) Length   498 a.a.
NCBI ID   WP_001040656.1    Uniprot ID   -
Organism   Escherichia coli strain TW10598     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3005873..3017369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA01_RS14485 tusA 3006120..3006365 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  EXA01_RS14490 zntA 3006467..3008665 (-) 2199 WP_000106551.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EXA01_RS14495 yhhN 3008739..3009365 (-) 627 WP_000964718.1 lysoplasmalogenase -
  EXA01_RS14500 yhhM 3009506..3009865 (+) 360 WP_000042895.1 DUF2500 domain-containing protein -
  EXA01_RS14505 yhhL 3009868..3010137 (-) 270 WP_001311191.1 DUF1145 family protein -
  EXA01_RS14510 rsmD 3010127..3010723 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EXA01_RS14515 pilA 3010873..3012369 (+) 1497 WP_001040656.1 signal recognition particle-docking protein FtsY Machinery gene
  EXA01_RS14520 ftsE 3012372..3013040 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EXA01_RS14525 ftsX 3013033..3014091 (+) 1059 WP_001042020.1 permease-like cell division protein FtsX -
  EXA01_RS14530 rpoH 3014336..3015190 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EXA01_RS14535 livJ 3015461..3016564 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EXA01_RS14540 panM 3016764..3017147 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54699.51 Da        Isoelectric Point: 4.1468

>NTDB_id=305740 EXA01_RS14515 WP_001040656.1 3010873..3012369(+) (pilA) [Escherichia coli strain TW10598]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=305740 EXA01_RS14515 WP_001040656.1 3010873..3012369(+) (pilA) [Escherichia coli strain TW10598]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAACCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378