Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA04_RS20805 Genome accession   NZ_CP035832
Coordinates   4253229..4253669 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 025542-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4248229..4258669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA04_RS20785 aroP 4249227..4250600 (+) 1374 WP_072133621.1 aromatic amino acid transporter AroP -
  EXA04_RS20790 ampE 4250643..4251497 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  EXA04_RS20795 ampD 4251494..4252045 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXA04_RS20800 nadC 4252133..4253026 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXA04_RS20805 pilA 4253229..4253669 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  EXA04_RS20810 pilB 4253679..4255064 (+) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  EXA04_RS20815 hofC 4255054..4256256 (+) 1203 WP_000157234.1 protein transport protein HofC -
  EXA04_RS20820 guaC 4256291..4257334 (-) 1044 WP_001217338.1 GMP reductase -
  EXA04_RS20825 - 4257490..4257534 (-) 45 WP_120795372.1 protein YacM -
  EXA04_RS20830 coaE 4257559..4258179 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=305500 EXA04_RS20805 WP_000360904.1 4253229..4253669(+) (pilA) [Escherichia coli strain 025542-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=305500 EXA04_RS20805 WP_000360904.1 4253229..4253669(+) (pilA) [Escherichia coli strain 025542-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment