Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EXA04_RS00685 Genome accession   NZ_CP035832
Coordinates   137282..137950 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain 025542-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 132282..142950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA04_RS00655 yicC 132301..133164 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  EXA04_RS00660 rph 133291..134007 (+) 717 WP_001247093.1 ribonuclease PH -
  EXA04_RS00665 pyrE 134073..134714 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  EXA04_RS00670 slmA 134751..135347 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EXA04_RS00675 dut 135454..135912 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EXA04_RS00680 coaBC 135890..137110 (-) 1221 WP_000050155.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EXA04_RS00685 radC 137282..137950 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  EXA04_RS00690 rpmB 138167..138403 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EXA04_RS00695 rpmG 138424..138591 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EXA04_RS00700 mutM 138689..139498 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EXA04_RS00705 coaD 139537..140016 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EXA04_RS00710 waaA 140024..141301 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EXA04_RS00715 rfaQ 141714..142772 (+) 1059 WP_000360412.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=305443 EXA04_RS00685 WP_001298959.1 137282..137950(+) (radC) [Escherichia coli strain 025542-1]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=305443 EXA04_RS00685 WP_001298959.1 137282..137950(+) (radC) [Escherichia coli strain 025542-1]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496