Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA06_RS21105 Genome accession   NZ_CP035822
Coordinates   4364437..4364877 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain WS4437A-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4359437..4369877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA06_RS21080 coaE 4359927..4360547 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  EXA06_RS21085 - 4360572..4360616 (+) 45 WP_120795372.1 protein YacM -
  EXA06_RS21090 guaC 4360772..4361815 (+) 1044 WP_001217338.1 GMP reductase -
  EXA06_RS21095 hofC 4361850..4363052 (-) 1203 WP_000157266.1 protein transport protein HofC -
  EXA06_RS21100 pilB 4363042..4364427 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  EXA06_RS21105 pilA 4364437..4364877 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  EXA06_RS21110 nadC 4365080..4365973 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EXA06_RS21115 ampD 4366061..4366612 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EXA06_RS21120 ampE 4366609..4367463 (+) 855 WP_001412484.1 beta-lactamase regulator AmpE -
  EXA06_RS21125 aroP 4367506..4368879 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=305345 EXA06_RS21105 WP_000360904.1 4364437..4364877(-) (pilA) [Escherichia coli strain WS4437A-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=305345 EXA06_RS21105 WP_000360904.1 4364437..4364877(-) (pilA) [Escherichia coli strain WS4437A-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment